From 2007.igem.org

Revision as of 21:12, 5 June 2007 (view source) Samanthaliang (Talk | contribs) ← Older edit		Latest revision as of 18:06, 8 June 2007 (view source) Samanthaliang (Talk | contribs)
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-	NOT DONE YET...found an ALWNI site in Cre, use BglI instead	+	'''[rbs][ATG][lox][term][lox]''' <br>
-		+	'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
-	'''[rbs][ATG]''' <br>	+	'''using Bca1090 rbs site''' <br>
-	'''4 Different rbs variants (1106A, 1106B, 1128A, 1090)''' <br>	+
-	'''2 Different Cre variants (212 and 213)''' <br>	+
	----		----
-	Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br>	+	Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>	+	Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small) <br>
	Product is pBca9145-I716214. <br>		Product is pBca9145-I716214. <br>
-	Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br>	+	Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>	+	Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small) <br>
	Product is pBca9145-I716215. <br>		Product is pBca9145-I716215. <br>
-
-
-	Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large) <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
-	Product is pBca9145-I716216. <br>
-
-
-	Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large) <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
-	Product is pBca9145-I716217. <br>
-
-	Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br>
-	Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small) <br>
-	Product is pBca9145-I716218. <br>
-
-
-	Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br>
-	Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small)) <br>
-	Product is pBca9145-I716219. <br>
-
-
-	Digest pBca1101-Bca1128A  (BamHI/BglI, 1606+850+544bp, Large) <br>
-	Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small) <br>
-	Product is pBca9145-I716220. <br>
-
-
-	Digest pBca9145-Bca1090  (BamHI/BglI, 1538+553+9bp, Large) <br>
-	Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small)<br>
-	Product is pBca9145-I716221. <br>

---

Latest revision as of 18:06, 8 June 2007

[rbs][ATG][lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site

---

Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small)
Product is pBca9145-I716214.

Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small)
Product is pBca9145-I716215.