BerkiGEM2007-KristinConstructionFile2
From 2007.igem.org
(Difference between revisions)
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Biobricking of hemB | Biobricking of hemB | ||
PCR kd005 and kd006 on E.Coli K-12 MG1655 (1006 bp, Bg1II/XhoI) | PCR kd005 and kd006 on E.Coli K-12 MG1655 (1006 bp, Bg1II/XhoI) | ||
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kd005 Cloning of hemB ccaaaAGATCTatgacagacttaatccaacgc | kd005 Cloning of hemB ccaaaAGATCTatgacagacttaatccaacgc | ||
kd006 Cloning of hemB gctagCTCGAGttaGGATCCttaacgcagaatcttcttctc | kd006 Cloning of hemB gctagCTCGAGttaGGATCCttaacgcagaatcttcttctc | ||
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Latest revision as of 21:12, 6 June 2007
Biobricking of hemB PCR kd005 and kd006 on E.Coli K-12 MG1655 (1006 bp, Bg1II/XhoI) Sub into pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-I716153 ------------------------------------ kd005 Cloning of hemB ccaaaAGATCTatgacagacttaatccaacgc kd006 Cloning of hemB gctagCTCGAGttaGGATCCttaacgcagaatcttcttctc
