Waterloo

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<center><font size="6">[[Image:UWLogo.jpg]] University of Waterloo iGEM Team </font></center>
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<center> [[Image:UW_iGEMLogoHeader.png | 935px]] </center>
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<br>
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== Our Team ==
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{| style="border-spacing:0px; border-width:thin; border-color:black; border-style:solid"
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The UW iGEM team is a very interdisciplinary group. Our team members span the three faculties of Science, Mathematics and Engineering and include the programs of Biology, Biomedical Sciences, Computer Science, Bioinformatics, Computer Engineering, Electrical Engineering, Chemical Engineering, and Mathematical Physics at undergraduate and graduate levels. Even our professor advisors are cross-appointed to two other faculties. Our diverse backgrounds bring together a wide range of skills and ideas to the iGEM project. iGEM is giving us the opportunity to apply the skills learned in our lectures and labs to real life applications in molecular biology and biotechnology.
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|-
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| style="font-size:x-large; font-weight:bold; border-bottom-width:thin; border-bottom-color:black; border-bottom-style:solid; font-size:x-large; font-weight:bold; padding:5px; background-color:#FBCC30" | Our Team
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|-
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| style="padding:5px" | [[Image:Group photo mimi1.JPG|470px|left|thumb|Undergraduate Members]]  [[Image:UW_Advisors.jpg|407px|center|thumb|Faculty and Graduate Advisors]]
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|-
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| style="padding:5px" | The UW iGEM team is a very interdisciplinary group. Our team members span three faculties: Science, Mathematics, and Engineering, and represent a wide range of undergraduate programs: Biology, Biomedical Sciences, Biochemistry, Computer Science, Bioinformatics, Computer Engineering, Electrical Engineering, Chemical Engineering, Systems Design Engineering, and Mathematical Physics.
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==Abstract==
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Drawing on our diverse backgrounds, we bring a wide range of skills and modes of creative thinking to our iGEM project. The iGEM competition is providing us with an opportunity to become more familiar with the emerging field of synthetic biology in an engaging and fun atmosphere.  In addition to gaining experience in the design, construction, and analysis of genetic circuits, we are also meeting the challenge of bringing together a large, diverse group toward a common goal.
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==Background==
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===Binary Addition===
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When working in binary, only two digits are used: 0 and 1. Counting therefore proceeds as:
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# 1
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# 10
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# 11
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# 100
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# 101
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# 111
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etc.
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To add two binary numbers, the process is much the same as adding two decimal (ordinary) numbers, except that instead of carrying when two digits add to ten, carrying must be performed when two digits add to two. In other words, 0 + 1 adds to 1, but 1 + 1 adds to 0 with a carry of 1, which gives 10 (just as in decimal 1 + 9 would add to 0 with a carry of 1, to give 10).
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<br clear="all">
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===Half-Adder vs Full-Adder===
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{| style="border-spacing:0px; border-width:thin; border-color:black; border-style:solid; width:100%"
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Any construct designed to add two numbers will either be a half-adder or a full-adder. A half-adder can only add together two single digits, whereas a full-adder is needed to add two numbers of any greater length.
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|-
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| colspan="4" style="border-bottom-width:thin; border-bottom-color:black; border-bottom-style:solid; font-size:x-large; font-weight:bold; padding:5px; background-color:#FBCC30" | Our Project
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|-
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| colspan="4" style="padding:5px; font-size:large" | Abstract
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|-
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| colspan="2" style="vertical-align:top; border-bottom-width:thin; border-bottom-color:black; border-bottom-style:solid; padding:5px" | The goal of this project is to design a basic device for computing. Our idea was to reproduce a circuit element called a half adder with DNA, which takes in two 1-bit inputs, adds them, and outputs a sum and a carry. Our device responds to two inputs: red light and the chemical tetracycline. The input sensors control a set of genetic switches in order to carry out the computation and fluoresces green, red, or neither, depending on the outcome.
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For example, a half-adder could perform the addition 1 + 0 = 1, or 1 + 1 = 10, but it would take a full-adder to be able to perform 1100101 + 100101.
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Half adders are used as building blocks for full adders, which perform calculations similar to long addition but in binary. They are also an essential component in a device called the Arithmetic Logic Unit (ALU), a fundamental building block for the central processing unit in a modern computer. ALUs perform simple and complex operations such as bitwise logical operations and mathematical operations.  
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In terms of implementation, we say a half-adder accepts two inputs (the two digits to be summed) and returns two outputs (the "sum bit" and the "carry bit"). To add 1 + 1, the two inputs would each be 1, the sum bit would be 0, and the carry bit would be 1.
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The constructs for the half adder were built in parallel as well as the testing constructs. A future extension to this project would be to create a full adder. More information on each stage of the project is available below.
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A full-adder can be constructed by linking together multiple half-adders, with a slight modification: each half-adder must now accept, as an extra input, the carry bit from the previous half-adder in the chain. Each half-adder is responsible for adding one pair of corresponding digits from the two numbers to be added, ''and'' it must add to that the carry bit from the previous half-adder. The half-adder will output the resulting sum bit and carry bit, and the process will continue until all the digits have been added.
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| colspan="2" style="border-bottom-width:thin; border-bottom-color:black; border-bottom-style:solid; padding:5px; vertical-align:middle" | [[Image:Design schematic.jpg|thumb|center|475px|Schematic Design of the Biological Half-Adder]]
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|-
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| style="width:25%; border-right-width:thin; border-right-color:black; border-right-style:solid; font-size:large; text-align:left; padding:5px; background-color:#FBCC30" | [[Project | Project Design]] || style="width:25%; border-right-width:thin; border-right-color:black; border-right-style:solid; font-size:large; text-align:left; padding:5px; background-color:#FBCC30"  | [[Modelling | Mathematical Modelling]] || style="width:25%; border-right-width:thin; border-right-color:black; border-right-style:solid; font-size:large; text-align:left; padding:5px; background-color:#FBCC30" | [[Construction_and_Testing | Construction and Testing]] || style="width:25%; font-size:large; text-align:left; padding:5px; background-color:#FBCC30" | [[Future_Work | Future Work]]
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|-
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| style="vertical-align:top; border-right-width:thin; border-right-color:black; border-right-style:solid; padding:5px" |
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* Binary addition and boolean logic
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* Half-adder vs. full-adder designs
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* Biological half-adder implementation
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| style="vertical-align:top; border-right-width:thin; border-right-color:black; border-right-style:solid; padding:5px" |
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* Modelling the gene regulatory network
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* Simulation results
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| style="vertical-align:top; border-right-width:thin; border-right-color:black; border-right-style:solid; padding:5px" |
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* Strategy for half-adder construction
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* Testing constructs for device
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* Test execution plan
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* Submitted parts
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| style="vertical-align:top; padding:5px" |
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* Explanation of a full adder
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* Gene design for full adder
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* Implementation plan
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|}
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===DNA as Logic Gates===
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<br clear="all">
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==Project Details==
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{| style="border-spacing:0px; border-width:thin; border-color:black; border-style:solid; width:100%; text-align:center"
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===Inputs (stimuli and the genes used to detect them)===
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|-
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===Output (observable change and what it represents)===
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| style="font-size:x-large; font-weight:bold; font-size:x-large; font-weight:bold; padding:5px; background-color:#FBCC30; border-bottom-width:thin; border-bottom-color:black; border-bottom-style:solid; text-align:left" colspan="15" | Acknowledgements
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===Gene Diagram===
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|-
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| colspan="3" | [[Image:Fsf_logo.gif|150px]] || colspan="3" | [[Image:MEF_logo.gif|150px]] || colspan="3" | [[Image:SFF_Logo.gif|150px]] || colspan="3" | [[Image:WEEFLogo.jpg|150px]] || colspan="3" | [[Image:WatSEF_Logo.jpg|150px]]
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|-
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| colspan="3" | [http://www.science.uwaterloo.ca/fsf/index.html Faculty of Science Foundation ] || colspan="3" | [http://www.student.math.uwaterloo.ca/~mefcom/ Mathematics Endowment Fund ] || colspan="3" | [http://www.eng.uwaterloo.ca/~sff/ Sir Sanford Fleming Foundation] || colspan="3" | [http://www.weef.uwaterloo.ca/ Waterloo Engineering Endowment Fund] || colspan="3" | [http://www.science.uwaterloo.ca/~watsef/mainpage.html Waterloo Science Endowment Fund]
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|-
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| colspan="15" | &nbsp;
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|-
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| colspan="5" | [[Image:UW_EngFacLogo.PNG |150px]] || colspan="5" | [[Image:UW_SciFacLogo.PNG|150px]] || colspan="5" | [[Image:UW_MathFacLogo.PNG|150px]]
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|-
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| colspan="5" | [http://www.engineering.uwaterloo.ca University of Waterloo Faculty of Engineering] || colspan="5" | [http://www.science.uwaterloo.ca University of Waterloo Faculty of Science] || colspan="5" | [http://www.math.uwaterloo.ca University of Waterloo Faculty of Mathematics]
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|-
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| colspan="15" | &nbsp;
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|-
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| colspan="5" style="padding:5px" | [[Image:UW_Waterloocrest.PNG | 150px]] || colspan="10" style="text-align:left; padding:5px" | We would like to thank the following people for their support and guidance:
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* Dr. Trevor Charles
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* Dr. Barbara Moffatt
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* Dr. Joshua Neufeld
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|}
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==Testing/Results==
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<br clear="all">
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===Mathematical Model===
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<center>
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===Measurements===
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  [[Waterloo | Home ]] | [[Project | Project]] | [[Modelling | Mathematical Modelling]] | [[Construction_and_Testing | Construction and Testing]] | [[Future_Work | Future Work]]
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</center>
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==Extensions==
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===Full Adder===
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==Acknowledgements== (Sponsor logos)
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Latest revision as of 04:02, 27 October 2007

UW iGEMLogoHeader.png


Our Team
Undergraduate Members
Faculty and Graduate Advisors
The UW iGEM team is a very interdisciplinary group. Our team members span three faculties: Science, Mathematics, and Engineering, and represent a wide range of undergraduate programs: Biology, Biomedical Sciences, Biochemistry, Computer Science, Bioinformatics, Computer Engineering, Electrical Engineering, Chemical Engineering, Systems Design Engineering, and Mathematical Physics.

Drawing on our diverse backgrounds, we bring a wide range of skills and modes of creative thinking to our iGEM project. The iGEM competition is providing us with an opportunity to become more familiar with the emerging field of synthetic biology in an engaging and fun atmosphere. In addition to gaining experience in the design, construction, and analysis of genetic circuits, we are also meeting the challenge of bringing together a large, diverse group toward a common goal.


Our Project
Abstract
The goal of this project is to design a basic device for computing. Our idea was to reproduce a circuit element called a half adder with DNA, which takes in two 1-bit inputs, adds them, and outputs a sum and a carry. Our device responds to two inputs: red light and the chemical tetracycline. The input sensors control a set of genetic switches in order to carry out the computation and fluoresces green, red, or neither, depending on the outcome.

Half adders are used as building blocks for full adders, which perform calculations similar to long addition but in binary. They are also an essential component in a device called the Arithmetic Logic Unit (ALU), a fundamental building block for the central processing unit in a modern computer. ALUs perform simple and complex operations such as bitwise logical operations and mathematical operations.

The constructs for the half adder were built in parallel as well as the testing constructs. A future extension to this project would be to create a full adder. More information on each stage of the project is available below.

Schematic Design of the Biological Half-Adder
Project Design Mathematical Modelling Construction and Testing Future Work
  • Binary addition and boolean logic
  • Half-adder vs. full-adder designs
  • Biological half-adder implementation
  • Modelling the gene regulatory network
  • Simulation results
  • Strategy for half-adder construction
  • Testing constructs for device
  • Test execution plan
  • Submitted parts
  • Explanation of a full adder
  • Gene design for full adder
  • Implementation plan


Acknowledgements
Fsf logo.gif MEF logo.gif SFF Logo.gif WEEFLogo.jpg WatSEF Logo.jpg
[http://www.science.uwaterloo.ca/fsf/index.html Faculty of Science Foundation ] [http://www.student.math.uwaterloo.ca/~mefcom/ Mathematics Endowment Fund ] [http://www.eng.uwaterloo.ca/~sff/ Sir Sanford Fleming Foundation] [http://www.weef.uwaterloo.ca/ Waterloo Engineering Endowment Fund] [http://www.science.uwaterloo.ca/~watsef/mainpage.html Waterloo Science Endowment Fund]
 
UW EngFacLogo.PNG UW SciFacLogo.PNG UW MathFacLogo.PNG
[http://www.engineering.uwaterloo.ca University of Waterloo Faculty of Engineering] [http://www.science.uwaterloo.ca University of Waterloo Faculty of Science] [http://www.math.uwaterloo.ca University of Waterloo Faculty of Mathematics]
 
UW Waterloocrest.PNG We would like to thank the following people for their support and guidance:
  • Dr. Trevor Charles
  • Dr. Barbara Moffatt
  • Dr. Joshua Neufeld 


  Home  |  Project |  Mathematical Modelling |  Construction and Testing |  Future Work
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