Tokyo/New AHL check
From 2007.igem.org
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| - | === | + | __NOTOC__ |
| - | + | ===Objective: === | |
| + | To determine and compare the activity of the AHL used so far and that of the newly purchased AHL. | ||
| + | |||
===Samples: === | ===Samples: === | ||
| - | + | A4 placQI in pTrc99A (pos. con.) | |
<br>A4ΔP in pTrc99A (neg. con.) | <br>A4ΔP in pTrc99A (neg. con.) | ||
<br>A4 hybrid promoter in pBR322 | <br>A4 hybrid promoter in pBR322 | ||
===Procedure: === | ===Procedure: === | ||
| - | + | The old and the newly delivered AHL was used. | |
<br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | <br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | ||
<br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | <br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | ||
===Result & Conclusion: === | ===Result & Conclusion: === | ||
| + | The ability of the new AHL to activate the pomoters was almost the twice of that of old one. | ||
| + | |||
| + | == == | ||
| + | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Latest revision as of 05:17, 27 October 2007
Objective:
To determine and compare the activity of the AHL used so far and that of the newly purchased AHL.
Samples:
A4 placQI in pTrc99A (pos. con.)
A4ΔP in pTrc99A (neg. con.)
A4 hybrid promoter in pBR322
Procedure:
The old and the newly delivered AHL was used.
AHL assay Standard protocol
The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM.
Result & Conclusion:
The ability of the new AHL to activate the pomoters was almost the twice of that of old one.
