Paris/October 21

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(→Digestion Reactions)
 
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[[Paris/October 20|yesterday]] -- [[Paris/October 22|tomorrow]] <br>
== Miniprep ==
== Miniprep ==
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 +
The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.
== Transformation of Ligation products ==
== Transformation of Ligation products ==
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== Digestion Reactions ==
== Digestion Reactions ==
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Digestion Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=24%| Product Name
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|width=24%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| Size
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|width=34%| Description
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|width=1%|
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|width=1%|
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|width=1%|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D82
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|frt1-CmR-frt2 FI
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|P16
 +
|EcoRI
 +
|SpeI
 +
|
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|frt1-CmR-frt2 PCR product digested for use as a FI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D83
 +
|frt1-CmR-frt2 BI
 +
|P16
 +
|XbaI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2 PCR product digested for use as a BI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D84
 +
|frt1-CmR-frt2 BBi
 +
|P16
 +
|EcoRI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2 PCR product digested for cloning as a BB
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D96
 +
|frt1-CmR-frt2-T1T2 FI
 +
|P16
 +
|EcoRI
 +
|SpeI
 +
|
 +
|frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D97
 +
|frt1-CmR-frt2-T1T2 BI
 +
|P16
 +
|XbaI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D98
 +
|frt1-CmR-frt2-T1T2 BBi
 +
|P16
 +
|EcoRI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D95
 +
|PacI[pY-2C-IntC]SmaI
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|pY-2C-IntC plasmid
 +
|PacI
 +
|SmaI
 +
|
 +
|pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
 +
|
 +
|
 +
|
 +
|}

Latest revision as of 13:04, 22 October 2007

yesterday -- tomorrow

Contents

Miniprep

The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.

Transformation of Ligation products

Purification of PCR products

Digestion Reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB
D96 frt1-CmR-frt2-T1T2 FI P16 EcoRI SpeI frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
D97 frt1-CmR-frt2-T1T2 BI P16 XbaI PstI frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
D98 frt1-CmR-frt2-T1T2 BBi P16 EcoRI PstI frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
D95 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI SmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
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