Imperial/Wet Lab/Protocols
From 2007.igem.org
m (→Wet Lab: Protocols) |
|||
(62 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
+ | <br clear="all"> | ||
+ | |||
{{Template:IC07navmenu}} | {{Template:IC07navmenu}} | ||
__NOTOC__ | __NOTOC__ | ||
+ | <br clear="all"> | ||
= Wet Lab: Protocols = | = Wet Lab: Protocols = | ||
Below is a databank of all the protocols that have been used in our projects. | Below is a databank of all the protocols that have been used in our projects. | ||
Line 12: | Line 15: | ||
| | | | ||
<font size=2><font color=green>'''Protein Purification'''</font> <br> | <font size=2><font color=green>'''Protein Purification'''</font> <br> | ||
- | * ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and Results | + | * ''Purification of LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.5|Results]] |
- | * ''Purification of GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and Results | + | * ''Purification of GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.6|Results]] |
<br> | <br> | ||
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | <font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | ||
- | * ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and Results | + | * ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.1|Results]]<br> |
- | <br> | + | |
<font size=2><font color=green>'''Calibration Curve'''</font> <br> | <font size=2><font color=green>'''Calibration Curve'''</font> <br> | ||
- | * ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results | + | * ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.3|Results]] |
- | * ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results | + | * ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]] |
<br> | <br> | ||
<font size=2><font color=green>'''Chassis Protocols'''</font> <br> | <font size=2><font color=green>'''Chassis Protocols'''</font> <br> | ||
- | * '' | + | * ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]] |
- | * '' | + | * ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]] |
- | * '' | + | * ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]] |
- | * ''Vesicles Formation'' :[[Imperial/Wet_Lab/Protocols/VF1.1| | + | * ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]] |
+ | * ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br> | ||
+ | * ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]] | ||
<br> | <br> | ||
| | | | ||
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | <font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | ||
- | *''pT7 in vitro:'' | + | *''pT7 in vitro:'' [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.9|Results]] |
- | + | ||
- | + | ||
<br> | <br> | ||
- | '''Implementation''' | + | |
+ | <font size=2>'''Cell by Date'''</font> <br> | ||
+ | <font color=green>'''Implementation'''</font><br> | ||
+ | * ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.1|Results]] | ||
+ | * ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]] | ||
+ | <font color=green>'''Testing/Validation<br>'''</font> | ||
+ | *''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]] | ||
+ | * ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]] | ||
+ | *''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]] | ||
+ | *''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]] | ||
<br> | <br> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | <font size=2 | + | <font size=2>'''Infector Detector'''</font> <br> |
- | Implementation<br> | + | <font color=green>'''Implementation<br>'''</font> |
- | *''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [ | + | *''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]] |
- | * ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [ | + | * ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.2|Results]] |
- | Testing/Validation<br> | + | <font color=green>'''Testing/Validation<br>'''</font> |
- | * ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and Results | + | *''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID2.1|Results]] |
- | * ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and Results | + | * ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.7|Results]] |
+ | * ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID3.1|Results]] | ||
|} | |} | ||
<br clear="all"> | <br clear="all"> | ||
+ | |||
+ | |||
+ | <center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center> |
Latest revision as of 02:15, 27 October 2007
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
General Experiments | Testing Experiments |
Protein Purification
Calibration Curve
|
pT7 Characterisation
Cell by Date Testing/Validation
Infector Detector Testing/Validation |