Freiburg07/Gel Electrophoresis
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'''Gel Electrophoresis''' | '''Gel Electrophoresis''' | ||
- | + | ||
+ | TBE-Puffer (10×) | ||
1 M Tris-HCl<br> | 1 M Tris-HCl<br> | ||
| | ||
- | 1M | + | 1M Boric acid<br> |
| | ||
- | 25 mM EDTA< | + | 25 mM EDTA<br> |
<p><br> | <p><br> | ||
- | EtBr-Stock- | + | EtBr-Stock-Solution (10 000×) 5 |
- | mg/ml | + | mg/ml Ethidium bromide in H2O</p> |
<p><br> | <p><br> | ||
- | DNA- | + | DNA-loading-buffer (6×) |
- | 50% | + | 50% Glycerol<br> |
- | + | ||
- | + | ||
| | ||
- | 0.25% | + | 0.25% Bromphenol blue<br> |
- | + | | |
+ | 0.25% Xylencyanol<br> | ||
[https://2007.igem.org/Freiburg BACK] | [https://2007.igem.org/Freiburg BACK] |
Latest revision as of 08:35, 25 October 2007
Gel Electrophoresis
TBE-Puffer (10×)
1 M Tris-HCl
1M Boric acid
25 mM EDTA
EtBr-Stock-Solution (10 000×) 5
mg/ml Ethidium bromide in H2O
DNA-loading-buffer (6×)
50% Glycerol
0.25% Bromphenol blue
0.25% Xylencyanol
BACK