Freiburg07/Protein purification

From 2007.igem.org

(Difference between revisions)
 
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Gel filtration buffer:                                 
Gel filtration buffer:                                 
50 mM Tris×HCl pH=7,5<br>
50 mM Tris×HCl pH=7,5<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;150 mM NaCl<br>
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150 mM NaCl<br>
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<br>
[https://2007.igem.org/Freiburg BACK]
[https://2007.igem.org/Freiburg BACK]

Latest revision as of 09:15, 25 October 2007

Protein purification

Equilibration buffer:                                 50 mM Tris×HCl pH=7,5
1 M NaCl
Elution buffer:                                        Equilibration buffer
                                                             + 5 mM Chloramphenicol


Gel filtration buffer:                                50 mM Tris×HCl pH=7,5
                                                           150 mM NaCl

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