Princeton

From 2007.igem.org

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==Overview==
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<center>[[Image:PrincetonLogo2007.png|400x400px| 2007 logo]] [[Image:GroupPictureSmallcropped.jpg|750x400px|Group picture]]<br><br>
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===2007: [[Princeton/Project Description | A Lentivirus-Delivered, RNAi-Enhanced Logic Circuit for Cancer-Specific Detection and Destruction]] ===
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The [[Princeton/People | Princeton University iGEM 2007 team]], consisting of 10 undergraduate students, 2 high school students, and 8 instructors, envisions a paradigm shift in the way we envisage cancer cell targeting.
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=[[Princeton/Project Description | An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction]]=
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Using standard engineering practices, we have designed, and are in the process of implementing, a new system that utilizes RNA interference (RNAi) mechanisms to detect and destroy cancer cells in a tissue-specific manner. We will interface RNAi components together with promoter and repressor elements to form logic circuits, which will allow for the use of multiple criteria to further specify cancer targets. Additionally, we utilize mutant integrase to insert our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentiviral delivery mechanism, and ensuring that the system will not be inherited by future generations of the normal cells, thus preventing unintended proliferation of our construct. In doing so, we hope to minimize adverse effects to healthy cells, and thereby allow for more extensive and comprehensive cancer treatments.
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The [[Princeton/People | Princeton University iGEM 2007 team]], consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells.
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We are in the process of constructing our system, component by component, to throughly test each element and, where possible, revise the implementation of our constructs to improve effectiveness. [[Princeton/lab | Lab work]], including [[Princeton/lab/experimentation | experimentation]], [[Princeton/lab/bioinformatics | bioinformatics]], and [[Princeton/lab/simulation | simulations]], and [[Princeton/literature | literature]] have been used as feedback mechanisms to improve the implementation of the designed systems.
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Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. We have constructed many components of our system and are in the process of testing our plasmids in a proof of concept manner. Where possible, we will revise and improve upon our components based on mathematical modeling and experimental results.  
Please see the [[Princeton/overview | extended overview]] for more information.
Please see the [[Princeton/overview | extended overview]] for more information.
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<h3>Team Members</h3>
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==Previous work==
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*[[user:asichel|Andrew Sichel]] 
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*[[user:aswamina|Aparna Swaminathan]]
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*[[user:btsui|Bambi Tsui]]
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*[[user:cdehart|Caroline DeHart]]
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*[[user:dcooper|Devin Cooper]]
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*[[user:jmonk|Jon Monk]]
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*[[user:jchiu|Justine Chiu]]
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*[[user:katias|Katia Sherman]]
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*[[user:kmangal|Kush Mangal]]
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*[[user:nyuan|Neal Yuan]]
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*[[user:pcowgill|Paul Cowgill]]
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*[[user:vichij|Vichi Jagannathan]]
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===2006: Programmed differentiation of mouse embryonic stem cells using artificial signaling pathways===
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[[Image:Princeton_2006_projectsummary_heartandbrain.png|thumb|right|300px|Heart and brain]]
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<h3>Instructors</h3>
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Our vision is to develop reliable techniques for programmed tissue generation in mammalian systems. Our iGEM 2006 work encompasses artificial cell-cell signaling and signal processing, directed differentiation, pattern formation, modeling and precise cellular placement.
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*[[user:rweiss|Ron Weiss]] [mailto:rweiss@princeton.edu Contact Ron] [http://www.princeton.edu/~rweiss Website]
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*[[user:Cil|Cil Purnick]] [mailto:ppurnick@princeton.edu Contact Cil]
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*[[user:ilemisch|Ihor Lemischka]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43268&deptid=92 Website]
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*[[user:cry707|Christoph Schaniel]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43270&deptid=92 Website]
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*[[user:pguye|Patrick Guye]]
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*[[user:eskamoah|Noah Davidsohn]]
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*[[user:saurabhg|Saurabh Gupta]]
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A [https://2006.igem.org/wiki/index.php/Princeton:Project_Summary summary] of the project is available.
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<h3>Resources</h3>
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[[Princeton/literature | Literature on RNAi and Cancer]]
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<h3>Pictures</h3>
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[[Princeton/People | Our happy team]]
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|width=179.25px style="padding: 5px; background-color: #ffffaa; border: 2px solid #993300;" |
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<h3>Overview</h3>
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[[Princeton/overview | Our Project]]
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<h3>Lab Work</h3>
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[[Princeton/lab | Lab work]]
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<h3>Research</h3>
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[[Princeton/lab/experimentation | Experimentation]]
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<h3>Bioinformatics</h3>
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[[Princeton/lab/bioinformatics | Bioinformatics]]
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<h3>Simulations</h3>
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[[Princeton/lab/simulation | Simulations]]

Latest revision as of 03:48, 27 October 2007


2007 logo Group picture

An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction

The Princeton University iGEM 2007 team, consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells.

Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. We have constructed many components of our system and are in the process of testing our plasmids in a proof of concept manner. Where possible, we will revise and improve upon our components based on mathematical modeling and experimental results.

Please see the extended overview for more information.

Team Members

Instructors

Resources

Literature on RNAi and Cancer

Pictures

Our happy team

Overview

Our Project

Lab Work

Lab work

Research

Experimentation

Bioinformatics

Bioinformatics

Simulations

Simulations
Retrieved from "http://2007.igem.org/wiki/index.php/Princeton"