McGill

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Latest revision as of 03:57, 27 October 2007

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

In the Lab

Lab Notebook
Lab Protocols
Background Papers

The Team

Team Members
Mailing List

Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

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 <center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
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-== Project Overviews ==
-<u>'''Team 1 - Fluorescence Complimentation'''</U>
-This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence.  We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence.  These half proteins will then be attached to proteins that reversibly associate.<br>
+==  ==
+<center>[[Image:McGillIgem2007team.jpg|center|400px]]
+<html>
+<style>
-[[McGill/Team_1_More_Information|More Information]] <br>
+ul#list-nav {
-[[McGill/Team_1_Background_Papers|Background Papers]] <br>
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+margin:20px;
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-<u>'''Team 2 - Oscillator - Repressilator System'''</u>
+ul#list-nav li {
+display:inline
+}
-Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. The system uses a repressor (LacI) and an enhancer (LuxI) network to provide the oscillations, with the LacI gene coupled with a fluorescent reporter gene to visibly observe oscillations. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities. Extending the project, we plan to introduce the Repressilator three-gene oscillator (Elowitz et al.) side-by-side with the system.<br>
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-[[McGill/Team_2_More_Information|More Information]] <br>
+ul#list-nav li a:hover {
-[[McGill/Team_2_Background_Papers|Background Papers]] <br>
+background:#FFFFFF;
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+</style>
+<br>
+ <ul id="list-nav">
+<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
+<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
+<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
+<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
+<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
+<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
+</ul>
+</html>
+</center><br><br><br><br>
+== Project Overview ==
-<center>Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]</center><br>
+<u>'''Quorum-sensing coupled with the Repressilator'''</u>
+Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
+Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
+<center>
+[[Image:2gene oscillator.jpg|320px]]<br>
+</center><br>
+<br>
 {| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
 |-valign="top"
 |width=240px style="padding: 5px|
-==In the Lab==
+==[[Image:bacteria1.jpg]] In the Lab==
 [[McGill/Lab Notebook|Lab Notebook]]<br>
-[[McGill/Lab Protocols|Lab Protocols]]
+[[McGill/Lab Protocols|Lab Protocols]]<br>
+[[McGill/Team_2_Background_Papers|Background Papers]] <br>
 |width=240px style="padding: 5px|
-== The Team ==
-[[McGill/Team Roster| Team Roster]]<br>
+==[[Image:greenbulb.jpg]] The Team ==
+[[McGill/Team Roster| Team Members]]<br>
 [[McGill/Mailing List|Mailing List]]
-|}
+|width=240px style="padding: 5px|
+==[[Image:sine.jpg]] Pictures ==
+[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup]   (External) <br>
+[[McGill/RI meetup|Rhode Island Meetup]]<br>
+[[McGill/urc|McGill Research Conference]]<br>
+Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]

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