Valencia/Comparator
From 2007.igem.org
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{{Valencia/Cabecera}} | {{Valencia/Cabecera}} | ||
| - | During the brainstroming, an idea came up: mimetize an [[Valencia/Electronic comparator|electronic comparator]] on a biologcal device. It | + | During the brainstroming, an idea came up: mimetize an [[Valencia/Electronic comparator|electronic comparator]] on a biologcal device. It works with repressors, so it may sound familiar to most of us. |
'''What:''' | '''What:''' | ||
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Two different promoters that act as sensors of different inputs and two repressors that inhibit each other. | Two different promoters that act as sensors of different inputs and two repressors that inhibit each other. | ||
| - | #We have two promoters that 'sense' something: | + | #We have two promoters that 'sense' something: psensorA and psensorB |
#We have two promoters repressed: pLacI and ptet | #We have two promoters repressed: pLacI and ptet | ||
#We also have two CDS of the repressors: lacI and tetR | #We also have two CDS of the repressors: lacI and tetR | ||
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[[Image:VComparator-fluor.jpg|500px]] | [[Image:VComparator-fluor.jpg|500px]] | ||
| - | So, if we have equal amount of whatever A and B, there will be equal amounts of both repressors and, | + | So, if we have equal amount of whatever A and B, there will be equal amounts of both repressors and, so, equal amounts of CFP and YFP. If some input A is sensibly higher than the B, we will have more tetR that will inhibit ptet and we will have less YFP |
'''What for:''' | '''What for:''' | ||
| - | We would have a device that would clear the noise of a system. | + | We would have a device that would clear the noise of a system, (only reducing it to zero at infinite time). |
It can be used in other projects, as it implies only two genes and 4 promoters... | It can be used in other projects, as it implies only two genes and 4 promoters... | ||
Latest revision as of 15:06, 26 October 2007
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During the brainstroming, an idea came up: mimetize an electronic comparator on a biologcal device. It works with repressors, so it may sound familiar to most of us.
What:
Two inputs enter the system, a genetic circuit compares both signals and amplifies the higher one.
How:
So easy, I would be quite surprised if we don't find any drawback.
Two different promoters that act as sensors of different inputs and two repressors that inhibit each other.
- We have two promoters that 'sense' something: psensorA and psensorB
- We have two promoters repressed: pLacI and ptet
- We also have two CDS of the repressors: lacI and tetR
- And, finally, we have the outputs: CFP and YFP
The system would be:
So, if we have equal amount of whatever A and B, there will be equal amounts of both repressors and, so, equal amounts of CFP and YFP. If some input A is sensibly higher than the B, we will have more tetR that will inhibit ptet and we will have less YFP
What for:
We would have a device that would clear the noise of a system, (only reducing it to zero at infinite time). It can be used in other projects, as it implies only two genes and 4 promoters...
Problems:
- Will a gene driven by two different promoters work well enough?? ETH tried it with some results, why not give it a try?
- It's a small project, but it can be coupled to some other project...
- We still have to find some new BioBricks to build, as almost every single construction would be on the iGEM plate
Developed by: Arnau, Pablo and Javi
