June 16

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(Experiments)
 
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==Experiments ==
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''Back to [[Bangalore]]''
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*Check transformants --- Varun (Contamination in negative, lawn growth in rest)
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''Back to [[e-Notebook]]''
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*Image pLac cfp (9 AM onwards) (12,14 and 16 hrs)--- Navneet and Varun (Redo dilutions and image on 17th)
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== Experiments ==
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*FACS (9 AM onwards)--- Krishna
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*IPTG induction(pLac cfp) at 0,1,10,50,100,1000 uM- (X 2)
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* The transformants were checked and contamination was found in the negative control and lawn growth in rest of the samples. Thus imaging could not be done.
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*K12Z1 (10 PM) (X 2)--- Navneet (11 PM)
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''Inoculation''
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pLac cfp was inoculated with IPTG induction levels at 0,1,10,50,100,1000 uM along with K12Z1 as control.

Latest revision as of 07:17, 28 June 2007

Back to Bangalore

Back to e-Notebook

Experiments

  • The transformants were checked and contamination was found in the negative control and lawn growth in rest of the samples. Thus imaging could not be done.

Inoculation

pLac cfp was inoculated with IPTG induction levels at 0,1,10,50,100,1000 uM along with K12Z1 as control.