Ljubljana/biobricks

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<title>Company Name</title>
<title>Company Name</title>
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<link href="http://rokgaber.3host.biz/stylenew_zadaj.css" rel="stylesheet" type="text/css" />
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<link rel="stylesheet" href="/igem07/index.php?title=User:RGaber/stylesheet/stylenew_zadaj.css&action=raw&ctype=text/css" type="text/css" />
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     <div id="aboutred">
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     <div align="justify" id="aboutred">
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       <h3><span>Current Disease treatment</span></h3>
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       <h3><span>Biobricks</span></h3>
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       <p class="p1"><span><b>HIV is a typical retrovirus that upon entry into host cell reverse transcribes its RNA to DNA which integrates into the host genome. Viral genes are expressed as polyprotein that is cleaved into functional proteins by HIV protease. Viral coat is composed of lipids and two predominant proteins, gp41 and gp120, which are essential for binding onto the host cell receptor CD4. Several drugs were developed that target crucial stages of HIV entry into the host cell, its reverse transcription, integration and processing of the polyprotein. Current standard therapy consists of taking a combination of at least three different antiretroviral drugs.</b></span></p>
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       <p class="p1"><span><b>
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This year, our team has been the driving force of expanding the registry, creating no less than 77 parts. For our purpose we created, in addition to other parts, biobricks coding for individual domains of multi-domain proteins, involved in cellular signalling and antiviral response. These provide particular enrichment of the registry as they allow endless possibility to readily create novel chimeric proteins.
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</b></span></p>
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<div id="supportingText">
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<div align="justify">
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<div id="products">
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      <h3><span>Slovenian Team Jewelbox</span></h3>
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      <p class="p1"><span>Following parts were deposited into BioBrick database: </span></p><br>
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</div>
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<div></div></form>
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<small>
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<TABLE class='pgrouptable addborder' style='width: 600px;' cellpadding='0' cellspacing='0' border="0" style="background:#d0d2fb"><TR><TH colspan='2' style='width: 1em'  nowrap><A style='color:blue;text-decoration:none;'
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            href='http://partsregistry.org/Help:Availability_and _usefulness'></A><TH style='width: 7em;'>Name</TH><TH>Type<TH>Description<TH>Length<TH class='blank_col'>
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  <div id="supportingText">
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10080"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10080'>BBa_I712002</A></TD><TD>Coding</TD><TD>CCR5</TD><TD ALIGN='right'>1059</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10005"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10005'>BBa_I712003</A></TD><TD>Coding</TD><TD>CCR5-NUb</TD><TD ALIGN='right'>1194</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10004"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10004'>BBa_I712004</A></TD><TD>Regulatory</TD><TD>CMV promoter</TD><TD ALIGN='right'>654</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10032"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10032'>BBa_I712007</A></TD><TD>Tag</TD><TD>c-Myc-tag</TD><TD ALIGN='right'>30</TD><TD class  ='blank_col'></TD></FORM>
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<div id="products">
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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      <h3><span>Virus Life Cycle</span></h3>
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10033"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10033'>BBa_I712008</A></TD><TD>Tag</TD><TD>HA-tag</TD><TD ALIGN='right'>30</TD><TD class  ='blank_col'></TD></FORM>
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      <p class="p1"><span>HIV (human immunodeficiency virus) is a retrovirus, Its genome is composed of two single stranded RNA molecules. It has a gag/pol/env organization; gag genes (group specific antigen) code for structural proteins, env for proteins that build viral envelope, while pol genes are responsible for viral reproduction (they contain genes for reverse transcriptase, integrase and HIV protease).<br><br>
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HIV envelope consists of lipids and viral glycoproteins gp120 and gp41, which are crucial for binding of HIV to the host cell membrane and for entering into the cell. Inserted into the lipid bilayer are also other glycoproteins that guarantee firmness and protective function of the viral envelope. Gp120 binds to receptors (CD4) on the host cell surface, but additional co-receptors like chemokine receptors (CCR5, CXCR4) are also required for successful entry of HIV. Mutations in co-receptor genes can cause immunity – if HIV cannot enter host cells, HIV infection is prevented, and AIDS cannot develop.<br><br>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10162"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10162'>BBa_I712009</A></TD><TD>Coding</TD><TD>CD4 signal peptide</TD><TD ALIGN='right'>75</TD><TD class  ='blank_col'></TD></FORM>
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The characteristic retroviral enzyme is reverse transcriptase, which transcripts viral RNA into DNA. Only DNA can integrate into host cell genome – this is the crucial step in expressing viral proteins that are needed for assembly of new viral particles. Viral gag and gag/pol genes are expressed as polyprotein; until this polyprotein is cut into functional units, it exerts no biological function. Polyprotein clipping is done by HIV protease. The resulting polyprotein fragments represent functional enzymes and structural proteins.<br><br>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10242"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10242'>BBa_I712010</A></TD><TD>Coding</TD><TD>CD4 sequence without signal peptide</TD><TD ALIGN='right'>1299</TD><TD class  ='blank_col'></TD></FORM>
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Transcription of viral RNA into DNA and processing of the viral polyprotein are two most important steps in HIV replication cycle. These are thus obvious targets for HIV therapeutics. Inhibitors of reverse transcriptase and HIV protease are currently used to treat acute HIV infection.</a></span></p><br>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10243"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10243'>BBa_I712011</A></TD><TD>Coding</TD><TD>CUb (split ubiquitin system)</TD><TD ALIGN='right'>135</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10084"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10084'>BBa_I712012</A></TD><TD>Coding</TD><TD>TIR domain of TLR3</TD><TD ALIGN='right'>456</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10068"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10068'>BBa_I712015</A></TD><TD>Other</TD><TD>cleavage site for HIV-1 protease</TD><TD ALIGN='right'>42</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10246"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10246'>BBa_I712016</A></TD><TD>Other</TD><TD>myri + TEVP</TD><TD ALIGN='right'>57</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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      <h3><span>Current Disease Treatment</span></h3>
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10050"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10050'>BBa_I712017</A></TD><TD>Coding</TD><TD>Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.</TD><TD ALIGN='right'>1191</TD><TD class  ='blank_col'></TD></FORM>
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      <p class="p1">One of the first AIDS therapeutics were nucleotide or nucleoside analogues (NRTI – nucleoside-analogue reverse transcriptase inhibitors) – pseudosubstrates, that are during reverse transcription integrated into viral DNA instead of nucleosides and thus block the transcription. These drugs were superseded by non-nucleoside inhibitors (NNRTI) that could inhibit reverse transcriptase by binding into the alosteric site of the enzyme. The third type of drugs is a family of HIV protease inhibitors. In most cases specific inhibitors are very similar to protease substrates - the only difference is that because they cannot be cut, they block the active site by remaining bound into it.<br><br>
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Weakness of all these therapeutics is that they are very sensitive to HIV mutations – HIV can easily mutate and thus become drug resistant. A combination of drugs is used to minimize HIV's potential to develop resistance to each individual drug in the mixture. Some of the drugs induce mutations that have negative effect on the virulence and such drugs can be used in spite of developed resistance.<br><br>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10049"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10049'>BBa_I712019</A></TD><TD>Reporter</TD><TD>Firefly luciferase - luciferase from Photinus pyralis</TD><TD ALIGN='right'>1653</TD><TD class  ='blank_col'></TD></FORM>
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We still do not have a cure for AIDS that would be insensitive to HIV mutations. Our project presents new ways of potential AIDS therapeutics. Our approaches can be considered independent of HIV mutations. We have set up a few of biological ambushes; if HIV manages to avoid them, we presume that it would not be able to infect the cell anyway.</p>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10052"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10052'>BBa_I712020</A></TD><TD>Coding</TD><TD>Homo sapiens caspase 3</TD><TD ALIGN='right'>834</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10200"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10200'>BBa_I712021</A></TD><TD>Coding</TD><TD>Interferon beta</TD><TD ALIGN='right'>564</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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    </div>
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10163"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10163'>BBa_I712022</A></TD><TD>Coding</TD><TD>T7 RNA polymerase with NLS</TD><TD ALIGN='right'>2673</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10248"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10248'>BBa_I712024</A></TD><TD>Coding</TD><TD>CD4</TD><TD ALIGN='right'>1374</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10244"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10244'>BBa_I712026</A></TD><TD>Tag</TD><TD>myristoilation site</TD><TD ALIGN='right'>21</TD><TD class  ='blank_col'></TD></FORM>
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    <div id="support">
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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      <h3><span>Classes of Antiretroviral Drugs</span></h3>
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10006"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10006'>BBa_I712027</A></TD><TD>Composite</TD><TD>CMV + CCR5-NUb</TD><TD ALIGN='right'>1854</TD><TD class  ='blank_col'></TD></FORM>
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      <p class="p1">Antiretroviral drugs are mostly inhibitors of different stages in HIV life cycle. They are targeted at different enzymes or events that are typical for HIV infection – entry of the virus into the cell, reverse transcription, polyprotein cleavage... and are divided into seven main classes (REFERENCA!):<br>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10062"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10062'>BBa_I712028</A></TD><TD>Coding</TD><TD>CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence</TD><TD ALIGN='right'>733</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10109"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10109'>BBa_I712029</A></TD><TD>Composite</TD><TD>CMV-myc-CXCR4-NUb</TD><TD ALIGN='right'>1887</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10110"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10110'>BBa_I712030</A></TD><TD>Composite</TD><TD>CMV-CXCR4-NUb</TD><TD ALIGN='right'>1851</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10304"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10304'>BBa_I712031</A></TD><TD>Intermediate</TD><TD>CMVp-CD4-HA-CUb</TD><TD ALIGN='right'>2219</TD><TD class  ='blank_col'></TD></FORM>
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</p>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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    </div>
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10348"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10348'>BBa_I712033</A></TD><TD>Intermediate</TD><TD>CMVp - myristoilation site</TD><TD ALIGN='right'>681</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10350"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10350'>BBa_I712034</A></TD><TD>Intermediate</TD><TD>CMVp - myristoilation site - HIV protease cleavage site</TD><TD ALIGN='right'>731</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10300"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10300'>BBa_I712035</A></TD><TD>Composite</TD><TD>CMVp-CD4-CUb</TD><TD ALIGN='right'>2175</TD><TD class  ='blank_col'></TD></FORM>
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    <div id="development">
+
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
-
      <h3><span>Development</span></h3>
+
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10343"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10343'>BBa_I712037</A></TD><TD>Composite</TD><TD>CMVp - CD4 - CUb - mCherry - NLS</TD><TD ALIGN='right'>2914</TD><TD class  ='blank_col'></TD></FORM>
-
      <p class="p1">Lorem ipsum dolor sit amet, <a href="#">consetetur sadipscing elitr, sed diam nonumy</a> eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
 
-
      <p class="p2">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
-
      <p class="p3">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. </p>
+
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10344"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10344'>BBa_I712038</A></TD><TD>Composite</TD><TD>CMVp - CD4 - CUb - GFP</TD><TD ALIGN='right'>2901</TD><TD class  ='blank_col'></TD></FORM>
-
      <p class="p4">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
 
-
      <p class="p5">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore.</p>
+
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10346"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10346'>BBa_I712039</A></TD><TD>Intermediate</TD><TD>CMVp - HA - CD4 - CUb - GFP</TD><TD ALIGN='right'>2945</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10301"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10301'>BBa_I712040</A></TD><TD>Intermediate</TD><TD>CMVp-CD4-HIVcleavageSite</TD><TD ALIGN='right'>2084</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10319"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10319'>BBa_I712041</A></TD><TD>Composite</TD><TD>CMVp - CD4 - HIV protease cleavage site - mCherryNLS</TD><TD ALIGN='right'>2823</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10324"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10324'>BBa_I712043</A></TD><TD>Composite</TD><TD>CMVp - myristoilation site - HIV protease cleavage site - T7RNAP - NLS</TD><TD ALIGN='right'>3410</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10332"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10332'>BBa_I712044</A></TD><TD>Composite</TD><TD>CMVp - myristoilation site - HIV protease cleavage site - mCherry - NLS</TD><TD ALIGN='right'>1470</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10334"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10334'>BBa_I712045</A></TD><TD>Composite</TD><TD>CMVp - myristoilation site - HIV protease cleavage site - GFP</TD><TD ALIGN='right'>1457</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10321"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10321'>BBa_I712046</A></TD><TD>Composite</TD><TD>CMVp - HA - CD4 - HIV protease cleavage site - GFP</TD><TD ALIGN='right'>2854</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10312"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10312'>BBa_I712048</A></TD><TD>Composite</TD><TD>CMV + CD4 with ha tag + TIR (TLR3)</TD><TD ALIGN='right'>2542</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10241"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10241'>BBa_I712049</A></TD><TD>Composite</TD><TD>CMV + Myc + CCR5 + TIR(TLR3)</TD><TD ALIGN='right'>2183</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10250"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10250'>BBa_I712050</A></TD><TD>Composite</TD><TD>CMV promoter - RBS - T7 RNA polymerase - NLS</TD><TD ALIGN='right'>3340</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10207"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10207'>BBa_I712054</A></TD><TD>Reporter</TD><TD>pT7-mCer</TD><TD ALIGN='right'>772</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10335"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10335'>BBa_I712055</A></TD><TD>Composite</TD><TD>myri - TEVP - mCherry - NLS</TD><TD ALIGN='right'>796</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10336"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10336'>BBa_I712056</A></TD><TD>Composite</TD><TD>myri - TEVP - GFP</TD><TD ALIGN='right'>783</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10338"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10338'>BBa_I712057</A></TD><TD>Composite</TD><TD>myri - TEVP - T7 RNAP - NLS</TD><TD ALIGN='right'>2736</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10339"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10339'>BBa_I712058</A></TD><TD>Composite</TD><TD>CMVp - myri - TEVP - GFP</TD><TD ALIGN='right'>1443</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10340"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10340'>BBa_I712059</A></TD><TD>Composite</TD><TD>CMVp - myri - TEVP - T7 RNAP - NLS</TD><TD ALIGN='right'>3396</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10204"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10204'>BBa_I712064</A></TD><TD>Composite</TD><TD>pT7-IFNbeta</TD><TD ALIGN='right'>616</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10164"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10164'>BBa_I712069</A></TD><TD>Coding</TD><TD>RBS-T7 RNA polymerase with NLS</TD><TD ALIGN='right'>2678</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10251"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10251'>BBa_I712070</A></TD><TD>Composite</TD><TD>T7 promoter - RBS -T7 RNA polymerase with NLS</TD><TD ALIGN='right'>2732</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10071"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10071'>BBa_I712073</A></TD><TD>Device</TD><TD>CMV-Cas3</TD><TD ALIGN='right'>1494</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10177"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10177'>BBa_I712074</A></TD><TD>Regulatory</TD><TD>T7 promoter (strong promoter from T7 bacteriophage)</TD><TD ALIGN='right'>46</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10180"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10180'>BBa_I712076</A></TD><TD>Coding</TD><TD>CMV promoter - Myc tag - CCR5  (shortened)</TD><TD ALIGN='right'>1608</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10182"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10182'>BBa_I712077</A></TD><TD>Coding</TD><TD>TEV protease (N-terminal half)</TD><TD ALIGN='right'>357</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10184"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10184'>BBa_I712078</A></TD><TD>Coding</TD><TD>TEV protease (C-terminal half)</TD><TD ALIGN='right'>375</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10193"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10193'>BBa_I712079</A></TD><TD>Coding</TD><TD>CMV promoter - Myc tag - CCR5  (shortened) - TEV N</TD><TD ALIGN='right'>1973</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10198"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10198'>BBa_I712080</A></TD><TD>Coding</TD><TD>CMV promoter - Myc tag - CCR5 (shortened) - TLR3 TIR</TD><TD ALIGN='right'>2072</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10211"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10211'>BBa_I712082</A></TD><TD>Coding</TD><TD>CMV promoter - CD4 (shortened)</TD><TD ALIGN='right'>1907</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10214"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10214'>BBa_I712083</A></TD><TD>Composite</TD><TD>CMV promoter - CD4 (shortened) - TEV C</TD><TD ALIGN='right'>2290</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10215"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10215'>BBa_I712084</A></TD><TD>Composite</TD><TD>CMV promoter - CD4 (shortened) - TLR3 TIR</TD><TD ALIGN='right'>2371</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10293"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10293'>BBa_I712085</A></TD><TD>Composite</TD><TD>CMV - CD4(shortened) - Cub</TD><TD ALIGN='right'>2048</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10294"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10294'>BBa_I712086</A></TD><TD>Composite</TD><TD>CMV - CD4(shortened) - Cub - CherryNLS</TD><TD ALIGN='right'>2646</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10297"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10297'>BBa_I712087</A></TD><TD>Composite</TD><TD>CMV - CD4(shortened) - Cub - T7polNLS</TD><TD ALIGN='right'>4727</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10299"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10299'>BBa_I712088</A></TD><TD>Composite</TD><TD>CMV - CD4(shortened) - Cub - GFP</TD><TD ALIGN='right'>2774</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10306"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10306'>BBa_I712090</A></TD><TD>Composite</TD><TD>CMVp-CD4</TD><TD ALIGN='right'>2034</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10345"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10345'>BBa_I712564</A></TD><TD>Composite</TD><TD>CMV + fLuc</TD><TD ALIGN='right'>2313</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10314"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10314'>BBa_I712666</A></TD><TD>Composite</TD><TD>CMV + Myc + CCR5</TD><TD ALIGN='right'>1755</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10333"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10333'>BBa_I712667</A></TD><TD>Coding</TD><TD>HIV protease</TD><TD ALIGN='right'>303</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10337"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10337'>BBa_I712668</A></TD><TD>Composite</TD><TD>CMV - HIV protease</TD><TD ALIGN='right'>963</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10341"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10341'>BBa_I712669</A></TD><TD>Composite</TD><TD>CMV - mCer</TD><TD ALIGN='right'>1380</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10353"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10353'>BBa_I712670</A></TD><TD>Intermediate</TD><TD>CMV-HA</TD><TD ALIGN='right'>690</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10355"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10355'>BBa_I712671</A></TD><TD>Intermediate</TD><TD>CMV-c-myc</TD><TD ALIGN='right'>690</TD><TD class  ='blank_col'></TD></FORM>
 +
 
 +
<form method="post" action="/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2007&amp;group=iGEM2007_Ljubljana" enctype="multipart/form-data">
 +
<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="10363"  /><input type="hidden" name="pgroup" value="iGEM2007"  /><input type="hidden" name="group" value="igem2007_ljubljana"  /><TR><TD class='cell_white'>&nbsp;<TD class='cell_white'>&nbsp;<TD><A class='noul_link' href='view.cgi?part_id=10363'>BBa_I712672</A></TD><TD>Composite</TD><TD>CMV-IFNbeta</TD><TD ALIGN='right'>1224</TD><TD class  ='blank_col'></TD></FORM></TABLE></FORM><BR>
 +
        <!-- end content -->
 +
 
 +
</small>
 +
 
 +
   
     </div>
     </div>
 +
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<a href="https://2007.igem.org/Ljubljana/finalsystem">Performance of the Final Functional Systems</a>       
<a href="https://2007.igem.org/Ljubljana/finalsystem">Performance of the Final Functional Systems</a>       
|<a href="https://2007.igem.org/Ljubljana">Home</a>|
|<a href="https://2007.igem.org/Ljubljana">Home</a>|
-
<a href="https://2007.igem.org/Ljubljana/biobricks">Methods</a>
+
<a href="https://2007.igem.org/Ljubljana/methods">Methods</a>
</div>
</div>
   </div>
   </div>
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<div id="ldiscussion">
<div id="ldiscussion">
-
<ul><li><a class="one" href="https://2007.igem.org/Ljubljana/glossary">Terms & References</a>&nbsp; </li>
+
<ul><li><a class="one" href="https://2007.igem.org/Ljubljana/glossary">Glossary & References</a>&nbsp; </li>
<li><a class="one" href="https://2007.igem.org/Ljubljana/acknowledgements">Acknowledgements</a>&nbsp; </li>
<li><a class="one" href="https://2007.igem.org/Ljubljana/acknowledgements">Acknowledgements</a>&nbsp; </li>
         </ul>
         </ul>

Latest revision as of 18:52, 23 November 2007

Company Name

Biobricks

This year, our team has been the driving force of expanding the registry, creating no less than 77 parts. For our purpose we created, in addition to other parts, biobricks coding for individual domains of multi-domain proteins, involved in cellular signalling and antiviral response. These provide particular enrichment of the registry as they allow endless possibility to readily create novel chimeric proteins.

Slovenian Team Jewelbox

Following parts were deposited into BioBrick database:


NameTypeDescriptionLength
  BBa_I712002CodingCCR51059
  BBa_I712003CodingCCR5-NUb1194
   BBa_I712004RegulatoryCMV promoter654
  BBa_I712007Tagc-Myc-tag30
  BBa_I712008TagHA-tag30
  BBa_I712009CodingCD4 signal peptide75
  BBa_I712010CodingCD4 sequence without signal peptide1299
  BBa_I712011CodingCUb (split ubiquitin system)135
  BBa_I712012CodingTIR domain of TLR3456
  BBa_I712015Othercleavage site for HIV-1 protease42
  BBa_I712016Othermyri + TEVP57
  BBa_I712017CodingChemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.1191
  BBa_I712019ReporterFirefly luciferase - luciferase from Photinus pyralis1653
  BBa_I712020CodingHomo sapiens caspase 3834
  BBa_I712021CodingInterferon beta564
  BBa_I712022CodingT7 RNA polymerase with NLS2673
  BBa_I712024CodingCD41374
  BBa_I712026Tagmyristoilation site21
  BBa_I712027CompositeCMV + CCR5-NUb1854
  BBa_I712028CodingCherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence733
  BBa_I712029CompositeCMV-myc-CXCR4-NUb1887
  BBa_I712030CompositeCMV-CXCR4-NUb1851
  BBa_I712031IntermediateCMVp-CD4-HA-CUb2219
  BBa_I712033IntermediateCMVp - myristoilation site681
  BBa_I712034IntermediateCMVp - myristoilation site - HIV protease cleavage site731
  BBa_I712035CompositeCMVp-CD4-CUb2175
  BBa_I712037CompositeCMVp - CD4 - CUb - mCherry - NLS2914
  BBa_I712038CompositeCMVp - CD4 - CUb - GFP2901
  BBa_I712039IntermediateCMVp - HA - CD4 - CUb - GFP2945
  BBa_I712040IntermediateCMVp-CD4-HIVcleavageSite2084
  BBa_I712041CompositeCMVp - CD4 - HIV protease cleavage site - mCherryNLS2823
  BBa_I712043CompositeCMVp - myristoilation site - HIV protease cleavage site - T7RNAP - NLS3410
  BBa_I712044CompositeCMVp - myristoilation site - HIV protease cleavage site - mCherry - NLS1470
  BBa_I712045CompositeCMVp - myristoilation site - HIV protease cleavage site - GFP1457
  BBa_I712046CompositeCMVp - HA - CD4 - HIV protease cleavage site - GFP2854
  BBa_I712048CompositeCMV + CD4 with ha tag + TIR (TLR3)2542
   BBa_I712049CompositeCMV + Myc + CCR5 + TIR(TLR3)2183
  BBa_I712050CompositeCMV promoter - RBS - T7 RNA polymerase - NLS3340
  BBa_I712054ReporterpT7-mCer772
  BBa_I712055Compositemyri - TEVP - mCherry - NLS796
  BBa_I712056Compositemyri - TEVP - GFP783
  BBa_I712057Compositemyri - TEVP - T7 RNAP - NLS2736
  BBa_I712058CompositeCMVp - myri - TEVP - GFP1443
  BBa_I712059CompositeCMVp - myri - TEVP - T7 RNAP - NLS3396
  BBa_I712064CompositepT7-IFNbeta616
  BBa_I712069CodingRBS-T7 RNA polymerase with NLS2678
  BBa_I712070CompositeT7 promoter - RBS -T7 RNA polymerase with NLS2732
  BBa_I712073DeviceCMV-Cas31494
  BBa_I712074RegulatoryT7 promoter (strong promoter from T7 bacteriophage)46
   BBa_I712076CodingCMV promoter - Myc tag - CCR5 (shortened)1608
  BBa_I712077CodingTEV protease (N-terminal half)357
  BBa_I712078CodingTEV protease (C-terminal half)375
  BBa_I712079CodingCMV promoter - Myc tag - CCR5 (shortened) - TEV N1973
  BBa_I712080CodingCMV promoter - Myc tag - CCR5 (shortened) - TLR3 TIR2072
  BBa_I712082CodingCMV promoter - CD4 (shortened)1907
  BBa_I712083CompositeCMV promoter - CD4 (shortened) - TEV C2290
  BBa_I712084CompositeCMV promoter - CD4 (shortened) - TLR3 TIR2371
  BBa_I712085CompositeCMV - CD4(shortened) - Cub2048
  BBa_I712086CompositeCMV - CD4(shortened) - Cub - CherryNLS2646
  BBa_I712087CompositeCMV - CD4(shortened) - Cub - T7polNLS4727
  BBa_I712088CompositeCMV - CD4(shortened) - Cub - GFP2774
  BBa_I712090CompositeCMVp-CD42034
  BBa_I712564CompositeCMV + fLuc2313
  BBa_I712666CompositeCMV + Myc + CCR51755
   BBa_I712667CodingHIV protease303
   BBa_I712668CompositeCMV - HIV protease963
   BBa_I712669CompositeCMV - mCer1380
  BBa_I712670IntermediateCMV-HA690
  BBa_I712671IntermediateCMV-c-myc690
  BBa_I712672CompositeCMV-IFNbeta1224

Retrieved from "http://2007.igem.org/wiki/index.php/Ljubljana/biobricks"