McGill

From 2007.igem.org

(Difference between revisions)
(→Project Overview)
(→Image:sine.jpg Pictures)
 
(17 intermediate revisions not shown)
Line 4: Line 4:
{| cellspacing="2px" cellpadding="5" border="0" style="padding: 0; width: 935px; color: #000000; background-color: #ffffff;"
{| cellspacing="2px" cellpadding="5" border="0" style="padding: 0; width: 935px; color: #000000; background-color: #ffffff;"
| width=750px style="padding: 20px; background-color: #ffffff; border: 4px solid #FF0000;" |
| width=750px style="padding: 20px; background-color: #ffffff; border: 4px solid #FF0000;" |
-
<center>[[Image:McGillIgem2007team.jpg]]</center>
 
-
== Project Overview ==
+
== ==
 +
<center>[[Image:McGillIgem2007team.jpg|center|400px]]
 +
<html>
 +
<style>
-
<u>'''Quorum-sensing coupled with the Repressilator'''</u>
+
ul#list-nav {
 +
list-style:none;
 +
margin:20px;
 +
padding:0;
-
Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, expressing a Cyan fluorescent protein when turned on for visualization. This simple relaxation oscillator makes a robust system of oscillations that can act as a 'pace maker' for more complicated oscillating systems. Quorum-sensing coupling between the systems is achieved with a diffusible artificial inducer (AI) protein made from LuxI which couples when produced, to a constitutively expressed gene in the system LuxR which can turn 'on' the system by promoting the synthesis of Lac from pLux. As Lac accumulates, it acts to turn 'off' the system by repressing the pLac controlling the synthesis of LuxI itself. Also, once AI is produced, it can easily diffuse to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of oscillating cells. <br>
+
}
-
This year we've taken this system even further...  <u>[[McGill/Project Overview|learn more]]</u><br><br>
+
ul#list-nav li {
-
[[McGill/Team_2_More_Information|Animated System]] <br>
+
display:inline
-
[[McGill/Project Evaluation|Project Evaluation]] <br>
+
}
-
[[McGill/Parts|Parts/Biobricks]]<br><br>
+
-
[[McGill/Project Description|Project Description]]<br>
+
ul#list-nav li a {
-
[[McGill/Team_2_Background_Papers|Background Papers]] <br><br>
+
text-decoration:none;
-
<center>
+
padding:10px 0;
-
[[Image:repress_graph.jpg]]</center>
+
width:130px;
 +
background:#DB231E;
 +
color:#eee;
 +
float:left;
 +
text-align:center;
 +
border-left:1px solid #fff;
 +
}
 +
ul#list-nav li a:hover {
 +
background:#FFFFFF;
 +
color:#000
 +
}
 +
</style>
 +
<br>
 +
<ul id="list-nav">
 +
<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
 +
<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
 +
<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
 +
<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
 +
<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
 +
<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
 +
</ul>
 +
</html>
 +
</center><br><br><br><br>
-
<center>Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]</center><br>
+
== Project Overview ==
 +
<u>'''Quorum-sensing coupled with the Repressilator'''</u>
 +
 +
Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator. 
 +
 +
Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
 +
<center>
 +
[[Image:2gene oscillator.jpg|320px]]<br>
 +
</center><br>
 +
<br>
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
|-valign="top"
|-valign="top"
Line 32: Line 67:
[[McGill/Lab Notebook|Lab Notebook]]<br>
[[McGill/Lab Notebook|Lab Notebook]]<br>
[[McGill/Lab Protocols|Lab Protocols]]<br>
[[McGill/Lab Protocols|Lab Protocols]]<br>
 +
[[McGill/Team_2_Background_Papers|Background Papers]] <br>
Line 46: Line 82:
[[McGill/RI meetup|Rhode Island Meetup]]<br>
[[McGill/RI meetup|Rhode Island Meetup]]<br>
[[McGill/urc|McGill Research Conference]]<br>
[[McGill/urc|McGill Research Conference]]<br>
 +
 +
Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]

Latest revision as of 03:57, 27 October 2007

Banner 1.gif

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Contents

McGillIgem2007team.jpg






Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

2gene oscillator.jpg



Bacteria1.jpg In the Lab

Lab Notebook
Lab Protocols
Background Papers


Greenbulb.jpg The Team

Team Members
Mailing List

Sine.jpg Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

Retrieved from "http://2007.igem.org/wiki/index.php/McGill"