PennState/Project/Diauxie/DiauxieElimination

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'''Test for Diauxie Elimination'''
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To test for diauxie elimination, constitutively expressed xylR transcriptional regulator is added following the promoter test constructs. The increased level of xylR and CRP* in the cell should activate the natural chromosomal promoter for xylR, thus causing xylose metabolization even in the presence of glucose, but necessarily in the presence of xylose.
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<html><img src="http://openwetware.org/images/e/e6/Dtest.jpg" id="logo" name="logo" height="150" width="550" alt="" /></html>
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A possible model for cells naturally growing and cells growing with diauxie elimination is shown below. Elimination of diauxie with the use of CRP* could require too much of a time delay before reaching maximum growth. In this case the promoter region would need to be altered to resemble the consensus promoter sequence while keeping the xylR binding site to maintain xylose dependence.
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<html>
<html>
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<img src="https://static.igem.org/mediawiki/2007/thumb/a/a1/Psuigem07diauxicgrowthcurves4.jpg/636px-Psuigem07diauxicgrowthcurves4.jpg"/></a>
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<img src="https://static.igem.org/mediawiki/2007/thumb/f/fa/Psuigem07curve2.jpg/633px-Psuigem07curve2.jpg" id="logo" name="logo" height="350" width="375" alt="" /></a>
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</html>
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<html>
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<!-- START OF LUC'S CODE -->
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<!-- START CSS: the following css needs to be on the page to style the podcast divs properly. Diagonal span background based on www.mezzoblue.com/zengarden/alldesigns/ -->
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<style type="text/css">
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.column {
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width:  200px;
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position: relative;
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display: block;
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float: left;
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clear: none;
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margin-right: 10px;
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}
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.podcast{
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position: relative;
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float: none;
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border: 1px dotted #c7cbce;
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height: 110px;
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background-color: #f1f1f1;
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clear: both;
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margin-bottom: 42px;
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font: 12px/1.4em "Lucida Grande", "Lucida Sans Unicode", Verdana, Tahoma, Arial, sans-serif;
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padding: 0;
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width: 175px;
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}
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.podcast h3{
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color: #3d3d3d;
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margin: .2em 0 .5em;
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padding: 0 0 0 4px;
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font-family: Verdana, sans-serif;
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font-size: 100%;
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}
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.podcast a:link, .podcast a:visited{
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text-decoration: none;
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color: #3d3d3d;
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}
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.podcast a:hover {
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color: #aaaaff;
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}
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a:hover.screenshot {
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}
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.podcast .screenshot {
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float: left;
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position: relative;
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clear: none;
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display: block;
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margin: 0px 0px 0px 16px;
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}
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.podcast .screenshot img {
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background: #ffffff;
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border: 1px solid #c7cbce;
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padding: 4px;
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}
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.podcast .screenshot span {
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position: absolute;
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bottom:0px;
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right: 1px;
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padding: 4px 20px 4px 26px;
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background: url(https://static.igem.org/mediawiki/2007/0/09/Podcast-download-bg.gif) no-repeat;
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font-size: 0.8em;
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line-height: 1;
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.podcast .screenshot span a{
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padding: 0 0 0 0.5em;
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}
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.podcast .description {
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width: 220px;
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}
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.screenshot img:hover {
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border: 1px solid #aaaaff;
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}
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</style></html>
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<html>
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<!-- PODCAST HTML: the following html structures the information for each podcast. Each podcast entry is contained in its own podcast div, which are arranged into two columns by two column divs.  The last column must be cleared.-->
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<div class="column">
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<div class="podcast">
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<!-- this is where you need to post the link to where it will go (the second one)-->
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Diauxie','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Introduction
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie"
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<img src="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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</div><div class="column">
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<div class="podcast">
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<!-- this is where you need to post the link to where it will go (the second one)-->
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/PromoterTest" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Diauxie/PromoterTest','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Promoter Test Constructs
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/PromoterTest"
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<img src="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg" width="133" height="100" alt="" />  
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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</div>
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<div class="column"><div class="podcast">
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<!-- this is where you need to post the link to where it will go (the second one)-->
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/DiauxieElimination" ="window.popup_player_258600 = window.open('http://openwetware.org/images/c/c9/XylRdimer1.png','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Eliminating Diauxie
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/DiauxieElimination"
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<img src="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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</div>
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<div class="column"><div class="podcast">
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<!-- this is where you need to post the link to where it will go (the second one)-->
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<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/FinalConstructs" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Diauxie/FinalConstructs','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
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Final Constructs
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</a></h3>
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<div class="screenshot">
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<!-- little white box thing --> <a rel="enclosure" href="https://2007.igem.org/PennState/Project/Diauxie/FinalConstructs"
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<img src="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg" width="133" height="100" alt="" />
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</a>
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<span class="download">
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<a href="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg">Image Link</a>
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</span>
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</div>
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</div>
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<div style="clear:both"></div>
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<!-- END OF LUC'S CODE -->
</html>
</html>

Latest revision as of 23:48, 26 October 2007

Test for Diauxie Elimination

To test for diauxie elimination, constitutively expressed xylR transcriptional regulator is added following the promoter test constructs. The increased level of xylR and CRP* in the cell should activate the natural chromosomal promoter for xylR, thus causing xylose metabolization even in the presence of glucose, but necessarily in the presence of xylose.


A possible model for cells naturally growing and cells growing with diauxie elimination is shown below. Elimination of diauxie with the use of CRP* could require too much of a time delay before reaching maximum growth. In this case the promoter region would need to be altered to resemble the consensus promoter sequence while keeping the xylR binding site to maintain xylose dependence.


Introduction

Image Link

Promoter Test Constructs

Image Link

Eliminating Diauxie

Image Link

Final Constructs

Image Link

Retrieved from "http://2007.igem.org/wiki/index.php/PennState/Project/Diauxie/DiauxieElimination"