BerkiGEM2007 WikiPlaying2

From 2007.igem.org

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   <p><a href="https://2007.igem.org/Berkeley_UC">&lt;&lt;&lt; Return to UC Berkeley iGEM 2007 </a></p>
   <p><a href="https://2007.igem.org/Berkeley_UC">&lt;&lt;&lt; Return to UC Berkeley iGEM 2007 </a></p>
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   <p> <a href="https://2007.igem.org/BerkiGEM2007Present4">&lt;&lt;Previous Section: Chassis</a> | <a href="https://2007.igem.org/BerkiGEM2007Present5">Next Section: Genetic Self-Destruct&gt;&gt;</a></p>
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   <p><a href="https://2007.igem.org/Berkeley_Individual_Contributions">&lt;&lt;Previous Section: Individual Contributions</a> | <a href="https://2007.igem.org/BerkiGEM2007_Resources">Next Section: Team Resources&gt;&gt;</a></p>
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<h1 align="center">Growth Control</h1>
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<h1 align="center">Team Notebooks</h1>
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<h2 align="center"><strong>Introduction</strong> </h2>
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<p align="justify"> To prevent chance of infection or unwanted proliferation after  hemoglobin production, we have engineered a genetic self-destruct  mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which kills the cell, but leaves it  physically intact. <br>
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  <h3>Team Notebooks</h3>
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<p><br>
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    <a href="https://2007.igem.org/John_Dueber_Notebook" title="John Dueber Notebook"> John Dueber's Notebook</a><br>
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    <a href="https://2007.igem.org/Christopher_Anderson_Notebook" title="Christopher Anderson Notebook"> Christopher Anderson's Notebook</a><br>
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    <a href="https://2007.igem.org/Farnaz_Nowroozi_Notebook" title="Farnaz Nowroozi Notebook"> Farnaz Nowroozi's Notebook</a><br>
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    <a href="https://2007.igem.org/Amin_Hajimorad_Notebook" title="Amin Hajimorad Notebook"> Amin Hajimorad's Notebook</a><br>
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    <a href="https://2007.igem.org/Rickey_Bonds_Notebook" title="Rickey Bonds Notebook"> Rickey Bonds' Notebook</a><br>
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<p align="justify">Growth control in our system is established by the incorporation  of a plasmid that can be triggered to translate a toxin. The toxins are  endonucleases or RNAses that destroy the genetic material within a  bacteria and thus prevent the bacteria from replicating. Throughout the  summer, we worked on several different constructs of an inducible toxin  and screened for the ones with the best phenotype. </p>
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<p align="center">&nbsp;</p>
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    <em>Keep your wiki notebooks,  sequencing/construction logs, and the registry updated!</em> </p>
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<h2 align="center"><strong>An Inducible Toxin</strong> </h2>
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<p align="justify"> Using a PBAD promoter, we constructed several variations  of inducible toxins, including the colicin DNAse CeaB, endonucleases  BamHI and BglII, and RNAse barnase. Additionally, ribosome binding site  libraries were used in order to increase the likelihood of finding a  construct that would exhibit no growth after being induced with  arabinose, but normal growth when uninduced. </p>
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    <a href="https://2007.igem.org/Arthur_Yu_Notebook" title="Arthur Yu Notebook"> Arthur Yu's 1337 Notebook</a><br>
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<p align="justify"><strong>I716408C:</strong> </p>
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    <a href="https://2007.igem.org/Austin_Day_Notebook" title="Austin Day Notebook"> Austin Day's Notebook</a><br>
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<p align="justify"><img src="https://static.igem.org/mediawiki/2007/3/3b/Berk-Figure-Barnase.png" alt="" name="" width="298" height="156" align="left"></p>
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    <a href="https://2007.igem.org/David_Tulga_Notebook" title="David Tulga Notebook"> David Tulga's Notebook</a><br>
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<p align="justify">We screened libraries of potential hits with a Tecan growth assay, and  in the end, the constructs that showed the desirable phenotype are the  constructs shown below (I716408C and I716462). When uninduced, the  cells show growth comparable to regular DH10B cells, but when induced, the growth plateaus as the cells lose their ability to replicate due to  RNA and DNA destruction.</p>
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    <a href="https://2007.igem.org/Kristin_Doan_Notebook" title="Kristin Doan Notebook"> Kristin Doan's Notebook</a><br>
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<p align="justify">&nbsp;</p>
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    <a href="https://2007.igem.org/Samantha_Liang_Notebook" title="Samantha Liang Notebook"> Samantha's Notebook (June - July 19, 2007</a><br>
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<p align="justify">&nbsp;</p>
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    <a href="https://2007.igem.org/Samantha_Liang_Notebook2" title="Samantha Liang Notebook2"> Samantha's Notebook (July 20, 2007 - present)</a><br>
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<p align="justify"><strong>I716462:</strong></p>
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    <a href="https://2007.igem.org/Vaibhavi_Umesh_Notebook" title="Vaibhavi Umesh Notebook"> Vaibhavi Umesh's Notebook</a><br>
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<p align="justify"><img src="https://static.igem.org/mediawiki/2007/3/38/Berk-Figure-BamHI.png" alt="" name="" width="299" height="125" align="left"></p>
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    <a href="https://2007.igem.org/Kristin_Fuller_Notebook" title="Kristin Fuller Notebook"> Kristin Fuller's Notebook</a><br>
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    <a href="https://2007.igem.org/Vincent_Parker_Notebook" title="Vincent Parker Notebook"> Vincent Parker's Notebook</a><br>
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    <a href="https://2007.igem.org/Nhu_Nguyen_Notebook" title="Nhu Nguyen Notebook"> Nhu Nguyen's Notebook</a><br>
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  <a href="https://2007.igem.org/Hannah_Cole_Notebook" title="Hannah Cole Notebook"> Hannah Cole's Notebook</a></p>
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<h2 align="center">Characterization of Growth Ability</h2>
 
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<h2 align="center">Phenotype of dead cells</h2>
 
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Latest revision as of 23:07, 26 October 2007

Untitled Document

<<< Return to UC Berkeley iGEM 2007

<<Previous Section: Individual Contributions | Next Section: Team Resources>>

Team Notebooks

Team Notebooks


John Dueber's Notebook
Christopher Anderson's Notebook
Farnaz Nowroozi's Notebook
Amin Hajimorad's Notebook
Rickey Bonds' Notebook


Keep your wiki notebooks, sequencing/construction logs, and the registry updated!


Arthur Yu's 1337 Notebook
Austin Day's Notebook
David Tulga's Notebook
Kristin Doan's Notebook
Samantha's Notebook (June - July 19, 2007
Samantha's Notebook (July 20, 2007 - present)
Vaibhavi Umesh's Notebook
Kristin Fuller's Notebook


Vincent Parker's Notebook
Nhu Nguyen's Notebook
Hannah Cole's Notebook

 

 

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