BerkiGEM2007-VaibhaviConstructionFile6

From 2007.igem.org

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<pre>
<pre>
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Biobrickification of composite Ptet + RFP (product = I716251)
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Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene
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Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L)
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Digest pBca 9145 – Bca1090 (AlwNI/BamHI, 1538 + 553, L)
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Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L)
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Sub into I716055 (AlwNI/BglII, 1519 + 868, S)
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Product is I716251
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Product is I716256
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No Oligos Needed
No Oligos Needed
</pre>
</pre>

Latest revision as of 20:49, 27 June 2007

Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene
---------------------------------------------------
Digest pBca 9145 – Bca1090		(AlwNI/BamHI, 1538 + 553, L)
Sub into I716055			(AlwNI/BglII, 1519 + 868, S)
Product is I716256
---------------------------------------------------
No Oligos Needed
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