NYMU Taipei/Lab Notes/2007 12 12
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
* pOmpC + EYFP culture | * pOmpC + EYFP culture | ||
| - | ** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin | + | ** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004) |
| + | * pLacIQ + OmpRBS + GFP | ||
| + | ** re-culture pLacIQ in liquid medium (Kan+) | ||
| + | ** re-culture GFP in liquid medium () | ||
| + | ** re-culture EYFP in liquid medium () | ||
Latest revision as of 04:22, 13 December 2007
- pOmpC + EYFP culture
- 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004)
- pLacIQ + OmpRBS + GFP
- re-culture pLacIQ in liquid medium (Kan+)
- re-culture GFP in liquid medium ()
- re-culture EYFP in liquid medium ()
