From 2007.igem.org

Revision as of 23:37, 2 July 2007 (view source) Ayu (Talk | contribs) ← Older edit		Latest revision as of 23:38, 2 July 2007 (view source) Ayu (Talk | contribs)
Line 1:		Line 1:
	<pre>		<pre>
-	construct ptet/rbs/wbbL/rbs/neuS in lo copy	+	construct stuff
-	digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest	+	digest I716009 cm/rbs/cytB5 (EcoRI, BamHI) 2063+1236, smaller
-	digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest	+	digest I716010 kan/rbs/cytB5red (EcoRI, BglII)   2944+1027, larger
-	Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy	+	Ligate them. product I716011 cm-rbs-cytB5-rbs-cytB5red
	</pre>		</pre>

---

Latest revision as of 23:38, 2 July 2007

construct stuff

digest I716009 cm/rbs/cytB5 (EcoRI, BamHI)  2063+1236, smaller
digest I716010 kan/rbs/cytB5red (EcoRI, BglII)   2944+1027, larger
Ligate them. product I716011 cm-rbs-cytB5-rbs-cytB5red