Melbourne/Lab Notebook

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[[Melbourne|<Back to team home page>]]  
[[Melbourne|<Back to team home page>]]  
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==Week 1==
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*Gas Vesicle lab notebook:
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===25 June 2007===
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** [[Melbourne/Lab GV Notebook|Gas Vesicles Notebook]]
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Prepared LB agar plates.
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*Blue photoreceptor notebook:
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** [[Melbourne/Lab BL Notebook|Blue Photoreceptor Notebook]]
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====Transformation====
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*Photoreceptor & control system lab notebooks:
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#resuspended the following from Registry plates:
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** [[Melbourne/Lab Notebook Weeks 1-4|Weeks 1-4]]
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**BBa_I15008 - ho1 (P2 21A)
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** [[Melbourne/Lab Notebook Weeks 5-8|Weeks 5-8]]
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**BBa_I15009 - PcyA (P2 21C)
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** [[Melbourne/Lab Notebook Weeks 9-12|Weeks 9-12]]
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**BBa_R0084 - OmpR positive promoter (P1 11H)
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** [[Melbourne/Lab Notebook Weeks 13-16|Weeks 13-16]]
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##Punctured foil with pipette tip.
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** [[Melbourne/Lab Notebook Weeks 17-18|Weeks 17-18]]
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##Resuspended in 15uL ddH2O.
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##Stored in-20 (after taking 1uL for transformation).
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#[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells with shorter incubationtimes as follows:
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**30min on ice after DNA addition
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**10min on ice after heat shock
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**30min at 37degrees with LB
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===26 June 2007===
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===27 June 2007===
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===28 June 2007===
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===29 June 2007===
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===30 June 2007===
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==Week 2==
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===2 July 2007===
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=Now=
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===3 July 2007===
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===4 July 2007===
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===5 July 2007===
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===6 July 2007===
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==Week 3==
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===9 July 2007===
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===10 July 2007===
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===11 July 2007===
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===12 July 2007===
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===13 July 2007===
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==Week 4==
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===16 July 2007===
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===17 July 2007===
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===18 July 2007===
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===19 July 2007===
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===20 July 2007===
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==Week 5==
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===23 July 2007===
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===24 July 2007===
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===25 July 2007===
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===26 July 2007===
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===27 July 2007===
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==Week 6==
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===30 July 2007===
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===31 July 2007===
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===1  Aug 2007===
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===2  Aug 2007===
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===3  Aug 2007===
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Latest revision as of 14:25, 26 October 2007

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Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne/Lab_Notebook"