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	=====Primary & secondary Reagents Required including controls=====		=====Primary & secondary Reagents Required including controls=====
	*[[Melb:Miniprep kit|Miniprep kit]]		*[[Melb:Miniprep kit|Miniprep kit]]
-	*TAE buffer if DNA intended for long term storage	+	*[[Melbourne/Secondary Reagent TAE|TAE Buffer]] if DNA intended for long term storage
	=====Method including controls=====		=====Method including controls=====
-	#	+	* Use Protocol included in the Kit.
-	#	+	*A centrifugation method is used.
-	#	+	*Modifications:
		+	In an effort to increase the yield of DNA, the following can be performed.
		+	**The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
		+	**The volume of bacterial culture used can be increased and centrifuged in two or more spins.
		+
	=====Equipement Required=====		=====Equipement Required=====
-	*Microfuge tubes	+	*[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]]
	*Pipettes and tips		*Pipettes and tips
	*Microfuge		*Microfuge

---

Latest revision as of 11:08, 29 September 2007

<Return to list of protocols> <Team home page>

• Applications:
  1. Isolation of plasmid DNA from Bacterial cells

• Time to complete protocol:
  • Lab time: 1.5hrs
  • Waiting time: 10min
• Approximate cost of materials: $0.00

Method from primary and secondary reagents

Primary & secondary Reagents Required including controls

• Miniprep kit
• TAE Buffer if DNA intended for long term storage

Method including controls

• Use Protocol included in the Kit.
• A centrifugation method is used.
• Modifications:

In an effort to increase the yield of DNA, the following can be performed.

• • The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
  • The volume of bacterial culture used can be increased and centrifuged in two or more spins.

Equipement Required

• microcentrifuge: 1.7ml
• Pipettes and tips
• Microfuge

References

• Patricia Illing 03:49, 3 July 2007 (EDT):Miniprep protocol 3/July/2007