July 1
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* Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | * Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM | ||
| - | i) for FACS (in | + | i) for FACS (in sets of three) |
| - | ii) for Microscopy ( | + | ii) for Microscopy (in sets of two). |
Latest revision as of 12:30, 3 July 2007
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Experiments
- pL.LuxI.C was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
- pL.LuxR.Y was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
- Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
i) for FACS (in triplicate for a particular inoculum volume)
ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL)
- Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
i) for FACS (in sets of three)
ii) for Microscopy (in sets of two).
