Wisconsin/Agarose Gel
From 2007.igem.org
< Wisconsin(Difference between revisions)
(→1.2% Agarose Gel preperation) |
|||
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | ===1.2% Agarose Gel | + | ===1.2% Agarose Gel preparation=== |
*8 wells | *8 wells | ||
*600mg Agarose | *600mg Agarose | ||
| Line 5: | Line 5: | ||
*1uL 10mg/ml EtBr | *1uL 10mg/ml EtBr | ||
| - | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | + | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. |
| + | |||
| + | *2uL DNA | ||
| + | *12uL ddH<sub>2</sub>O | ||
| + | *4uL 5x Loading Dye | ||
| + | *4uL DNA Ladder for reference | ||
| + | |||
| + | Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA. | ||
Latest revision as of 19:49, 4 July 2007
1.2% Agarose Gel preparation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.
- 2uL DNA
- 12uL ddH2O
- 4uL 5x Loading Dye
- 4uL DNA Ladder for reference
Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.
