BerkiGEM2007-AustinConstructionFile26
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Alpha: <br> | Alpha: <br> | ||
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)<br> | PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)<br> | ||
| - | Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br> | + | Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.<br> <br> |
Beta: <br> | Beta: <br> | ||
PCR CA1099F/G00101 on Part 9145-I716081 <br> | PCR CA1099F/G00101 on Part 9145-I716081 <br> | ||
| - | Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | + | Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion. <br> |
<br><br> | <br><br> | ||
Product is 9145-I716076 (Second best all around) | Product is 9145-I716076 (Second best all around) | ||
Latest revision as of 18:31, 5 July 2007
Alpha:
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion.
Product is 9145-I716076 (Second best all around)
