BerkiGEM2007-KristinConstructionFile13
From 2007.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
Composite part of RBS (pBca1101-1106A) + hemB | Composite part of RBS (pBca1101-1106A) + hemB | ||
| - | Digest pBca1101-1106A | + | Digest pBca1101-1106A (BamH1/XhoI, 2132+850, L) |
Sub into pBca9145-I716153 (Bg1II/XhoI, 2063+990, S) | Sub into pBca9145-I716153 (Bg1II/XhoI, 2063+990, S) | ||
Product is pBca9145-I716163 | Product is pBca9145-I716163 | ||
</pre> | </pre> | ||
Latest revision as of 17:10, 17 July 2007
Composite part of RBS (pBca1101-1106A) + hemB Digest pBca1101-1106A (BamH1/XhoI, 2132+850, L) Sub into pBca9145-I716153 (Bg1II/XhoI, 2063+990, S) Product is pBca9145-I716163
