Template:Paris PCR 0
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|style="background: #ffcccc;"| PCR Settings | |style="background: #ffcccc;"| PCR Settings | ||
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| - | |style="background: #ccccff;"| | + | |style="background: #ccccff;"| Buffer (5x) |
| - | |style="background: #ccffcc;" colspan = "2" |{{{ | + | |style="background: #ccffcc;" colspan = "2" |{{{Buffer}}} |
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|style="background: #ccccff;"| Expected size | |style="background: #ccccff;"| Expected size | ||
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|style="background: #ccccff;"| Annealing (°C) | |style="background: #ccccff;"| Annealing (°C) | ||
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| - | |style="background: #ccccff;"| | + | |style="background: #ccccff;"| MgCl<sub>2</sub> 10µM |
| - | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{MgCl2}}} |
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|style="background: #ccffcc;"| {{{Size}}} | |style="background: #ccffcc;"| {{{Size}}} | ||
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|style="background: #ccffcc;"| {{{Annealing}}} | |style="background: #ccffcc;"| {{{Annealing}}} | ||
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| - | |style="background: #ccccff;"| | + | |style="background: #ccccff;"| dNTP 10µM |
| - | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{dNTP}}} |
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|style="background: #ccccff;"| Success | |style="background: #ccccff;"| Success | ||
|- | |- | ||
|style="background: #ccccff;"| Time Elongation | |style="background: #ccccff;"| Time Elongation | ||
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|style="background: #ccccff;"| Oligo F 10µM | |style="background: #ccccff;"| Oligo F 10µM | ||
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|style="background: #ccffcc;"| {{{v_oligoF}}} | |style="background: #ccffcc;"| {{{v_oligoF}}} | ||
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| - | |style="background: # | + | |style="background: #ccffcc;"| {{{Success}}} |
|- | |- | ||
| - | |style="background: # | + | |style="background: #ccffcc;"| {{{Elongation}}} |
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|style="background: #ccccff;"| Oligo R 10µM | |style="background: #ccccff;"| Oligo R 10µM | ||
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|style="background: #ccffcc;"| {{{v_oligoR}}} | |style="background: #ccffcc;"| {{{v_oligoR}}} | ||
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| - | |style="background: # | + | |style="background: #ccccff;"| Image (click to enlarge) |
|- | |- | ||
| - | |style="background: # | + | |style="background: #ccccff;"| Number cycles |
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|style="background: #ccccff;"| Water | |style="background: #ccccff;"| Water | ||
| style="background: #ccffcc;" colspan = "2" |{{{water}}} | | style="background: #ccffcc;" colspan = "2" |{{{water}}} | ||
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| - | |style="background: # | + | |style="background: #ccffcc;" rowspan| [[Image:{{{Image}}}|30px]] |
|- | |- | ||
| + | |style="background: #ccffcc;"| {{{Cycles}}} | ||
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| - | |||
|style="background: #ccccff;"| Polymerase | |style="background: #ccccff;"| Polymerase | ||
| style="background: #ccffcc;" colspan = "2" |{{{pol}}} | | style="background: #ccffcc;" colspan = "2" |{{{pol}}} | ||
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| - | |style="background: # | + | |style="background: #ccccff;"| Band (0=ladder) |
|- | |- | ||
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| - | | | + | | |
|style="background: #ccccff;"| DNA | |style="background: #ccccff;"| DNA | ||
| style="background: #ccffcc;" colspan = "2" |{{{DNA}}} | | style="background: #ccffcc;" colspan = "2" |{{{DNA}}} | ||
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| + | |style="background: #ccffcc;"| {{{Band}}} | ||
|} | |} | ||
Latest revision as of 16:45, 18 July 2007
| PCR : {{{Title}}} | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | {{{Buffer}}} | Expected size | |||
| Annealing (°C) | MgCl2 10µM | {{{MgCl2}}} | {{{Size}}} | |||
| {{{Annealing}}} | dNTP 10µM | {{{dNTP}}} | Success | |||
| Time Elongation | Oligo F 10µM | {{{n_oligoF}}} | {{{v_oligoF}}} | {{{Success}}} | ||
| {{{Elongation}}} | Oligo R 10µM | {{{n_oligoR}}} | {{{v_oligoR}}} | Image (click to enlarge) | ||
| Number cycles | Water | {{{water}}} | [[Image:{{{Image}}}|30px]] | |||
| {{{Cycles}}} | Polymerase | {{{pol}}} | Band (0=ladder) | |||
| DNA | {{{DNA}}} | {{{Band}}} | ||||
