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Revision as of 16:32, 21 July 2007 (view source) Eismoustique (Talk | contribs) (→Plasmids: MiniPrep Products) ← Older edit		Revision as of 19:11, 22 July 2007 (view source) Eismoustique (Talk | contribs) (→Plasmids: MiniPrep Products) Newer edit →
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	|<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>		|<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>
	|Medium constitutive promoter in J61002 (clones 3 + 4)		|Medium constitutive promoter in J61002 (clones 3 + 4)
-	|	+	|MP11.3
	| 20/07/07		| 20/07/07
	|}		|}

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Revision as of 19:11, 22 July 2007

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock)

PCR products

PCR products
Number	Name	Length	Comments	Product Left ?	Date
P1	Lox71-FtsA-FtsZ-1	1537
P2	FtsZ-2	1088
P3	DGAT-1
P4	DGAT-2
P5	Lox66-DapAColi	985
P6	Lox66-DapASubtilis	979			17/07/07
P7	Lox71-FtsZ	1260	EcoR1 site not mutated		17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Product Left ?	Date
MP1	pJ23100 in J61002	Strong constitutive promoter in J61002 (clones 1 + 2)		15/07/07
MP2	pJ23107 in J61002	Medium constitutive promoter in J61002 (clones 1 + 2)	MP2.2	15/07/07
MP3	B0015 in pSB1AK3	Double terminator (clones 1 + 2)		16/07/07
MP4	I0500 in pSB2K3	AraC pBAD (clones 1 + 2)		16/07/07
MP5	BBa_B0030 in pSB1A2	Strong RBS (clones 1 + 2)		18/07/07
MP6	BBa_E0422 in pSB1A2	ECFP (RBS+LVA+Term) (clones 1 + 2)		18/07/07
MP7	BBa_E0241 in pSB1A2	PoPs to GFP converter (clones 1 + 2)		18/07/07
MP8	BBa_E0840 in pSB1A2	gfp tri-part; strong rbs (clones 1 + 2)		18/07/07
MP9	BBa_J61047 in pSB1A2	Cre ORF (clones 1 + 2)		18/07/07
MP10	BBa_I0500 in pSB2K3	AraC pBAD (clones 1 + 2)		20/07/07
MP11	BBa_pJ23107 in p61002	Medium constitutive promoter in J61002 (clones 3 + 4)	MP11.3	20/07/07

Digestion Products

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Description	Product Left	Date
D1	pJ23100	pJ23100	Spe1	Pst1	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert		17/07/07
D2	mRFP	pJ23100	Spe1	Pst1			17/07/07
D3	pJ23107	pJ23107	Spe1	Pst1	Vector with the J23107 promoter ready for insertion of a RBS/CDS insert		17/07/07
D4	mRFP	pJ23107	Spe1	Pst1			17/07/07
D5	pJ23100	pJ23100	EcoR1	Spe1	The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.		17/07/07
D6	pJ23107	pJ23107	EcoR1	Spe1	The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.		17/07/07
D7	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	EcoR1	Pst1			17/07/07
D8	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	Xba1	Pst1			17/07/07

Strains (glycerol stock)

Strains glycerol stock
Number	Name	Description	Date
S1	MG1655	Standard E.Coli strain
S2	Acinetobacter ADP1	Strain used for TG coloration test with Red Nile
S3	DH5alpha pKs::DGAT	Enzyme catalysing the transormation of DAG into TG
S4	BBa_pJ23100	Strong constitutive promoter in J61002 (clones 1 + 2)
S5	BBa_B0015	Double terminator (clones 1 + 2)
S6	BBa_pJ23107	Medium constitutive promoter in J61002 (clones 1 + 2)	19/07/07
S7	BBa_B0030	Strong RBS (clones 1 + 2)	19/07/07
S8	BBa_E0422	ECFP (RBS+LVA+Term) (clones 1 + 2)	19/07/07
S9	BBa_E0241	PoPs to GFP converter (clones 1 + 2)	19/07/07
S10	BBa_E0840	gfp tri-part; strong rbs (clones 1 + 2)	19/07/07
S11	BBa_J61047	Cre ORF (clones 1 + 2)	19/07/07
S12	W121		05/07/07
S13	BBa_I0500	pBAD AraC (clone 1+2)	20/07/07
S14	BBa_pJ23107	Medium constitutive promoter in J61002 (clone 3+4)	20/07/07

• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3