From 2007.igem.org

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	====Wed 18th July====		====Wed 18th July====
	* Digest GFP and mint mini preps and ran on gel		* Digest GFP and mint mini preps and ran on gel
-	* Biobrick [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] revived	+	* Biobrick [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] (ECFP) revived
	====Thu 19th July====		====Thu 19th July====
-	* Repeat digest of mini preps with more DNA	+	* Repeat digest of GFP and mint mini preps with more DNA
	====Fri 20th July====		====Fri 20th July====
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	* Revived Pseudamonas Fluorescens from freeze dried culture		* Revived Pseudamonas Fluorescens from freeze dried culture
	:: Used to retrieve frs and ech genes in vanillin pathway		:: Used to retrieve frs and ech genes in vanillin pathway
		+	* Prepare 4 mini preps of ECFP
		+
		+	====Mon 23rd July====
		+	* Ran gels for ECFP mini preps
		+	:: Digested using Xba1 and Prt1

---

Revision as of 13:25, 24 July 2007

https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG

Contents 1 Biobricks Revived 2 Lab Diary 2.1 Thu 12th July 2.2 Fri 13th July 2.3 Mon 16th July 2.4 Tue 17th July 2.5 Wed 18th July 2.6 Thu 19th July 2.7 Fri 20th July 2.8 Mon 23rd July

Biobricks Revived

This is a list of the biobricks we have revived from the registry

Brick	Description	Well	Used for
[http://partsregistry.org/Part:BBa_J04430 BBa_J04430]	IPTG induced GFP	Plate 2, 1L	Test biobrick revival process
[http://partsregistry.org/Part:BBa_E0241 BBa_E0241]	PoPS --> GFP	Plate 2, 15L	For use in dif experement 1
[http://partsregistry.org/Part:BBa_J45700 BBa_J45700]	Wintergreen Scent	Plate 3, 7B	Place into lacto bacillus
[http://partsregistry.org/Part:BBa_E0422 BBa_E0422]	fast degrading ECFP	Plate 1, 11G	For use in dif experement 1

Lab Diary

Thu 12th July

• PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])

Test biobrick revival process

Fri 13th July

• Alfalfa Seeds Planted (in Agar)

Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)

• Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] revived

Mon 16th July

• Begin DNA extraction from Bamboo

Test DNA extraction process from plants

• Prepared mini preps for GFP and mint genes

Tue 17th July

• Alfalfa Transferred to compost

• DNA containing GFP and mint genes extracted from E.Coli

Wed 18th July

• Digest GFP and mint mini preps and ran on gel
• Biobrick [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] (ECFP) revived

Thu 19th July

• Repeat digest of GFP and mint mini preps with more DNA

Fri 20th July

• Prepared GFP and winter green biobricks for sequencing
• Revived Pseudamonas Fluorescens from freeze dried culture

Used to retrieve frs and ech genes in vanillin pathway

• Prepare 4 mini preps of ECFP

Mon 23rd July

• Ran gels for ECFP mini preps

Digested using Xba1 and Prt1