Paris/Freezer
From 2007.igem.org
(Difference between revisions)
(→Plasmids: MiniPrep Products) |
David.bikard (Talk | contribs) (→Digestion Products) |
||
| Line 155: | Line 155: | ||
|width=5%| Enzyme 1 | |width=5%| Enzyme 1 | ||
|width=5%| Enzyme 2 | |width=5%| Enzyme 2 | ||
| - | |width= | + | |width=5%| Size |
| + | |width=35%| Description | ||
|width=5%| Product Left | |width=5%| Product Left | ||
|width=5%| Date | |width=5%| Date | ||
| Line 164: | Line 165: | ||
|Spe1 | |Spe1 | ||
|Pst1 | |Pst1 | ||
| + | | | ||
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | |Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | ||
| | | | ||
| Line 171: | Line 173: | ||
|<bbpart>mRFP dblTer</bbpart> | |<bbpart>mRFP dblTer</bbpart> | ||
|<bbpart>pJ23100</bbpart> | |<bbpart>pJ23100</bbpart> | ||
| + | | | ||
|Spe1 | |Spe1 | ||
|Pst1 | |Pst1 | ||
| Line 180: | Line 183: | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
| + | | | ||
|Spe1 | |Spe1 | ||
|Pst1 | |Pst1 | ||
| Line 189: | Line 193: | ||
|<bbpart>mRFP dblTer</bbpart> | |<bbpart>mRFP dblTer</bbpart> | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
| + | | | ||
|Spe1 | |Spe1 | ||
|Pst1 | |Pst1 | ||
| Line 198: | Line 203: | ||
|<bbpart>pJ23100</bbpart> | |<bbpart>pJ23100</bbpart> | ||
|<bbpart>pJ23100</bbpart> | |<bbpart>pJ23100</bbpart> | ||
| + | | | ||
|EcoR1 | |EcoR1 | ||
|Spe1 | |Spe1 | ||
| Line 207: | Line 213: | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
| + | | | ||
|EcoR1 | |EcoR1 | ||
|Spe1 | |Spe1 | ||
| Line 218: | Line 225: | ||
|EcoR1 | |EcoR1 | ||
|Pst1 | |Pst1 | ||
| + | | | ||
| | | | ||
| | | | ||
| Line 225: | Line 233: | ||
|Lox66 DapA E.coli | |Lox66 DapA E.coli | ||
|Lox66 DapA E.coli (PCR product) | |Lox66 DapA E.coli (PCR product) | ||
| + | | | ||
|Xba1 | |Xba1 | ||
|Pst1 | |Pst1 | ||
Revision as of 14:04, 23 July 2007
Contents |
PCR products
| PCR products | |||||
|---|---|---|---|---|---|
| Number | Name | Length | Comments | Product Left ? | Date |
| P1 | Lox71-FtsA-FtsZ-1 | 1537 | |||
| P2 | FtsZ-2 | 1088 | |||
| P3 | DGAT-1 | ||||
| P4 | DGAT-2 | ||||
| P5 | Lox66-DapAColi | 985 | |||
| P6 | Lox66-DapASubtilis | 979 | 17/07/07 | ||
| P7 | Lox71-FtsZ | 1260 | EcoR1 site not mutated | 17/07/07 | |
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Product Left ? | Date |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | 15/07/07 | |
| MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2) | MP2.2 | 15/07/07 |
| MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) | 16/07/07 | |
| MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | 16/07/07 | |
| MP5 | BBa_B0030 in pSB1A2 | Strong RBS (clones 1 + 2) | 18/07/07 | |
| MP6 | BBa_E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | 18/07/07 | |
| MP7 | BBa_E0241 in pSB1A2 | PoPs to GFP converter (clones 1 + 2) | 18/07/07 | |
| MP8 | BBa_E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) | 18/07/07 | |
| MP9 | BBa_J61047 in pSB1A2 | Cre ORF (clones 1 + 2) | 18/07/07 | |
| MP10 | BBa_I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | 20/07/07 | |
| MP11 | BBa_pJ23107 in p61002 | Medium constitutive promoter in J61002 (clones 3 + 4) | MP11.3 | 20/07/07 |
| MP12 | BBa_pJ23107 in p61002 | Medium constitutive promoter in J61002 (clones 3) | 23/07/07 | |
Digestion Products
| Digestion Products | ||||||||
|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | Date |
| D1 | pJ23100 | pJ23100 | Spe1 | Pst1 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | 17/07/07 | ||
| D2 | mRFP | pJ23100 | Spe1 | Pst1 | 17/07/07 | |||
| D3 | pJ23107 | pJ23107 | Spe1 | Pst1 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | 17/07/07 | ||
| D4 | mRFP | pJ23107 | Spe1 | Pst1 | 17/07/07 | |||
| D5 | pJ23100 | pJ23100 | EcoR1 | Spe1 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | 17/07/07 | ||
| D6 | pJ23107 | pJ23107 | EcoR1 | Spe1 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | 17/07/07 | ||
| D7 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | 17/07/07 | |||
| D8 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | 17/07/07 | |||
Strains (glycerol stock)
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Date | |
| S1 | MG1655 | Standard E.Coli strain | ||
| S2 | Acinetobacter ADP1 | Strain used for TG coloration test with Red Nile | ||
| S3 | DH5alpha pKs::DGAT | Enzyme catalysing the transormation of DAG into TG | ||
| S4 | BBa_pJ23100 | Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| S5 | BBa_B0015 | Double terminator (clones 1 + 2) | ||
| S6 | BBa_pJ23107 | Medium constitutive promoter in J61002 (clones 1 + 2) | 19/07/07 | |
| S7 | BBa_B0030 | Strong RBS (clones 1 + 2) | 19/07/07 | |
| S8 | BBa_E0422 | ECFP (RBS+LVA+Term) (clones 1 + 2) | 19/07/07 | |
| S9 | BBa_E0241 | PoPs to GFP converter (clones 1 + 2) | 19/07/07 | |
| S10 | BBa_E0840 | gfp tri-part; strong rbs (clones 1 + 2) | 19/07/07 | |
| S11 | BBa_J61047 | Cre ORF (clones 1 + 2) | 19/07/07 | |
| S12 | W121 | 05/07/07 | ||
| S13 | BBa_I0500 | pBAD AraC (clone 1+2) | 20/07/07 | |
| S14 | BBa_pJ23107 | Medium constitutive promoter in J61002 (clone 3+4) | 20/07/07 | |
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
