Paris/July 21
From 2007.igem.org
(Difference between revisions)
Eismoustique (Talk | contribs) (→Digestion reactions) |
David.bikard (Talk | contribs) (→Digestion reactions) |
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|EcoI | |EcoI | ||
|SpeI | |SpeI | ||
| - | | | + | |2096 |
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | |Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 25: | Line 25: | ||
|EcoI | |EcoI | ||
|SpeI | |SpeI | ||
| - | | | + | |58 |
| - | |J23100 promoter ready for | + | |J23100 promoter ready for use as a fw-insert |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D11 | | style="background: #ccffcc;" |D11 | ||
| Line 33: | Line 33: | ||
|EcoRI | |EcoRI | ||
|SpeI | |SpeI | ||
| - | | | + | |2096 |
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | |Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 41: | Line 41: | ||
|EcoRI | |EcoRI | ||
|SpeI | |SpeI | ||
| - | | | + | |58 |
|J23107 promoter ready for usage as a fw-insert | |J23107 promoter ready for usage as a fw-insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| Line 49: | Line 49: | ||
|SpeI | |SpeI | ||
|PstI | |PstI | ||
| - | | | + | |887 |
|mRFP RBS/CDS/T sequence, can it be used as a backward inser? | |mRFP RBS/CDS/T sequence, can it be used as a backward inser? | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
Revision as of 14:27, 23 July 2007
Digestion reactions
| Digestion Products | ||||||
|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | size | Description |
| D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 2096 | Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) |
| D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 58 | J23100 promoter ready for use as a fw-insert |
| D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 2096 | Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter |
| D12 | pJ23107 promoter fw-insert | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 58 | J23107 promoter ready for usage as a fw-insert |
| D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | 887 | mRFP RBS/CDS/T sequence, can it be used as a backward inser? |
| D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | Cre ORF extracted for usage as a bw-insert | |
| D15 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS | |
| D16 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning | |
| D17 | pSB1A2 E0422 vector | E0422 (MP6.1 & MP6.2) | EcoRI | PstI | pSB1A2 open vector for BioBrick cloning | |
| D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |
| D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |
| D20 | fw insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |
| D21 | fw insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |
