Paris/July 23

(Difference between revisions)

Revision as of 22:50, 23 July 2007

w121 culture launched on 22/07/07 did not grow ON: I had forgot to add DAP to LB-Erythro growth medium.

at 12h30, the following culture was launched:

The transduction seems to have worked: isolation of clones on LB and LB+DAP for verification.

Each of the digestion reactions that follow were performed as follows:

NEB3 buffer used for XbaI/PstI double digestion NEBEcoRI buffer used for both EcoRI/SpeI & EcoRI/PstI double digestion reactions

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	size	Description
D22	pSB1A2 open vector	BBa_J61047 (MP9.1 & MP9.2)	EcoI	PstI		Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
D23	AraC/pBad promoter fw-insert	BBa_I0500 (MP4.1 & MP4.2)	EcoI	SpeI		AraC/pBad promoter ready for use as a forward insert
D24	lox71-ftsZ bw-insert	lox71-ftsZ PCR product P7	XbaI	PstI		lox71-ftsZ(unmutated) ready for use as a backward insert
D25	eCFP bw-insert	BBa_E0422 (MP6.1 & MP6.2)	XbaI	PstI		eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
D26	PoPs to GFP converter	BBa_E0421 (MP7.1 & MP7.2)	XbaI	PstI		PoPs to GFP converter ready for use as a backward insert
D27	gfp-tripart	BBa_E0840 (MP8.1 & MP8.2)	XbaI	PstI		gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert

@@ Line 18: / Line 18: @@
 == Digestion reactions ==
+Each of the digestion reactions that follow were performed as follows:
+*20µL DNA solution (miniprep or PCR product)
+*5µL Buffer 10x
+*0.5µL BSA 100x
+*2µL of each of the 2 enzymes indicated
+*H20 qsp 50µL
+NEB3 buffer used for XbaI/PstI double digestion
+NEBEcoRI buffer used for both EcoRI/SpeI & EcoRI/PstI double digestion reactions
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