Paris/July 25

From 2007.igem.org

(Difference between revisions)
(→Digestions)
(→Digestions)
Line 26: Line 26:
|PstI
|PstI
|~950
|~950
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D29
 +
|RBS-DapAColi BB
 +
|P8 (RBS-DapAColi)
 +
|XbaI
 +
|PstI
 +
|~950
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D30
 +
|RBS-DapASubtilis BB
 +
|P9 (RBS-DapASubtilis)
 +
|EcoRI
 +
|PstI
 +
|~950
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D31
 +
|RBS-DapASubtilis BB
 +
|P9 (RBS-DapASubtilis)
 +
|XbaI
 +
|PstI
 +
|~950
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D32
 +
|Lox71-FtsA-FtsZ
 +
|P10 Lox71-FtsA-FtsZ
 +
|XbaI
 +
|PstI
 +
|~2600
|
|
|
|
|
|
|}
|}

Revision as of 10:53, 25 July 2007

Digestions

  • P8 (RBS-DapAColi) with XbaI & PstI for BW insert 
                                      EcoRI & PstI for BioBricks
  • P9 (RBS-DapASubtilis) with XbaI & PstI for BW insert 
                                            EcoRI & PstI for BioBricks
  • P10 (Lox71-FtsA-FtsZ) with XbaI & PstI for BW insert


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D28 RBS-DapAColi BB P8 (RBS-DapAColi) EcoRI PstI ~950
D29 RBS-DapAColi BB P8 (RBS-DapAColi) XbaI PstI ~950
D30 RBS-DapASubtilis BB P9 (RBS-DapASubtilis) EcoRI PstI ~950
D31 RBS-DapASubtilis BB P9 (RBS-DapASubtilis) XbaI PstI ~950
D32 Lox71-FtsA-FtsZ P10 Lox71-FtsA-FtsZ XbaI PstI ~2600
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