BerkiGEM2007-AustinConstructionFile39
From 2007.igem.org
(Difference between revisions)
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PCR AD0010F/AD0010R on I716063 (462 bp)<br> | PCR AD0010F/AD0010R on I716063 (462 bp)<br> | ||
| - | Digest PCR product (EcoR1/Nco1, 446/11/5, Largest)<br> | + | Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)<br> |
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br> | Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br> | ||
Ligation product is I716089<br> | Ligation product is I716089<br> | ||
Latest revision as of 01:07, 30 July 2007
PCR AD0010F/AD0010R on I716063 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)
Ligation product is I716089
