Paris/July 30
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preparation of the following PCR mix | preparation of the following PCR mix | ||
| - | 18x (12.5µL 2xreaction mix)= 225 | + | *18x (12.5µL 2xreaction mix)= 225 |
| - | 18x (0.5µL oligo O18 VF2 10µM)=9 | + | *18x (0.5µL oligo O18 VF2 10µM)=9 |
| - | 18x (0.5µL oligo O19 VR 10µM)=9 | + | *18x (0.5µL oligo O19 VR 10µM)=9 |
| - | 12µL H20+colony (10min 100°) | + | *12µL H20+colony (10min 100°) |
Revision as of 10:00, 30 July 2007
PCR screen
On colony PCRs performed to screen for correct ligation reactions (performed on 27/07/07):
10 clones of L1 ligation reactions, 6 clones of L6 ligation reactions
preparation of the following PCR mix
- 18x (12.5µL 2xreaction mix)= 225
- 18x (0.5µL oligo O18 VF2 10µM)=9
- 18x (0.5µL oligo O19 VR 10µM)=9
- 12µL H20+colony (10min 100°)
