Paris/July 30
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| - | == PCR screen == | + | == Colonies PCR screen == |
On colony PCRs performed to screen for correct ligation reactions (performed on 27/07/07): | On colony PCRs performed to screen for correct ligation reactions (performed on 27/07/07): | ||
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*12µL H20+colony (10min 100°) | *12µL H20+colony (10min 100°) | ||
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| + | == PCRs == | ||
Revision as of 12:02, 30 July 2007
Colonies PCR screen
On colony PCRs performed to screen for correct ligation reactions (performed on 27/07/07):
10 clones of L1 ligation reactions, 6 clones of L6 ligation reactions
preparation of the following PCR mix
- 18x (12.5µL 2xreaction mix)= 225
- 18x (0.5µL oligo O18 VF2 10µM)=9
- 18x (0.5µL oligo O19 VR 10µM)=9
- 12µL H20+colony (10min 100°)
