Paris/July 22
From 2007.igem.org
(Difference between revisions)
(→E.Coli pKS::DGAT) |
|||
| Line 22: | Line 22: | ||
* Observation: | * Observation: | ||
| - | [[Image: Paris/coli_dgat_07222007|center|900px]] | + | [[Image: Paris/coli_dgat_07222007.jpg|center|900px]] |
Revision as of 15:39, 30 July 2007
Digestion products
Purification of the digestion products of 21/07/07 purif. using the SV wizard gel cleanup system
elution of the purification products in 30-40microL H2O
Overnight cultures launched
The following ON cultures were launched:
- pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep
- w121 (from glycerol stock S12) in 5 ml LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media
I was unable to launch the following culture because of lack of a glycerol stock:
- pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep
E.Coli pKS::DGAT
We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See July 18).
- Observation:
- Interpretation:
We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences.
