Template:BerkiGEM2007 AustinSequencingFiles
From 2007.igem.org
(Difference between revisions)
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|ald244 || 7-26-07 || 9145-I716085 #2 cpcr || || g00101 || Can see link between dialpha and whole beta, looks perfect || [[Arkin_ald_seq-ald244 | ald244]] | |ald244 || 7-26-07 || 9145-I716085 #2 cpcr || || g00101 || Can see link between dialpha and whole beta, looks perfect || [[Arkin_ald_seq-ald244 | ald244]] | ||
|- | |- | ||
| - | |ald245 || | + | |ald245 || 7-30-07 || petDUET-I716091 || 1 || AD0010F || || [[Arkin_ald_seq-ald245 | ald245]] |
|- | |- | ||
| - | |ald246 || | + | |ald246 || 7-30-07 || petDUET-I716091 || 1 || AD0011F || || [[Arkin_ald_seq-ald246 | ald246]] |
|- | |- | ||
| - | |ald247 || | + | |ald247 || 7-30-07 || petDUET-I716093 || 2-1 || AD0010F || || [[Arkin_ald_seq-ald247 | ald247]] |
|- | |- | ||
| - | |ald248 || | + | |ald248 || 7-30-07 || petDUET-I716094 || 1-3 || AD0010F || || [[Arkin_ald_seq-ald248 | ald248]] |
|- | |- | ||
| - | |ald249 || | + | |ald249 || 7-30-07 || petDUET-I716094 || 1-3 || AD0011F || || [[Arkin_ald_seq-ald249 | ald249]] |
|- | |- | ||
|ald250 || || || || || || [[Arkin_ald_seq-ald250 | ald250]] | |ald250 || || || || || || [[Arkin_ald_seq-ald250 | ald250]] | ||
Revision as of 00:28, 31 July 2007
The numbers start at where the Arkin Wiki left off.
| Sample | Date | Plasmid | Clone | Primer | Result | File Link |
| ald123 | 6/6/07 | 9145-I716053 | N/A | ca998 | perfect | ald123 |
| ald124 | 6/6/07 | 9145-I716054 | N/A | ca998 | perfect | ald124 |
| ald125 | 6/6/07 | 9145-I716055 | #1 | ca998 | wrong | ald125 |
| ald126 | 6-7-07 | 9145-I716055 | #2 | ca998 | perfect | ald126 |
| ald127 | 6-7-07 | pBCA1101-bca9231 | ca998 | ald127 | ||
| ald128 | 6-9-07 | I716058 AKA:I716053m29 | #1 | ca998 | perfect, point muation in EcoR1 Site? | ald128 |
| ald129 | 6-9-07 | I716058 AKA:I716053m29 | #2 | ca998 | Deletion following mutation | ald129 |
| ald130 | 6-9-07 | I716058 AKA:I716053m29 | #3 | ca998 | perfect, point muation in EcoR1 Site? | ald130 |
| ald131 | 6-9-07 | I716059 AKA:I716054m108 | #1 | ca998 | Perfect | ald131 |
| ald132 | 6-9-07 | I716059 AKA:I716054m108 | #2 | ca998 | Perfect | ald132 |
| ald133 | 6-9-07 | I716059 AKA:I716054m108 | #3 | ca998 | Perfect | ald133 |
| ald134 | 6-14-07 | 9145-Bca1154 | #1 | ca998 | (Revised: Mixed plasmid) silent point mutation, Gln caa -> cag, %15.33 -> %28.92 | ald134 |
| ald135 | 6-14-07 | 9145-Bca1154 | #2 | ca998 | 2 point mutations, but were close calls. Probably correct | ald135 |
| ald136 | 6-14-07 | 9145-Bca1150 | #1 | ca998 | perfect | ald136 |
| ald137 | 6-14-07 | 9145-Bca1150 | #2 | ca998 | wrong | ald137 |
| ald138 | 6-14-07 | pTrc-I716061 | #1 | ca998 | wrong oligo... | ald138 |
| ald139 | 6-14-07 | pTrc-I716061 | #2 | ca998 | wrong oligo... | ald139 |
| ald140 | 6-14-07 | pTrc-I716061 | #3 | ca998 | wrong oligo... | ald140 |
| ald141 | 6-14-07 | pTrc-I716061 | #4 | ca998 | wrong oligo... | ald141 |
| ald142 | 6-14-07 | 9145-I716062 | #1 | ca998 | perfect | ald142 |
| ald143 | 6-14-07 | 9145-I716062 | #2 | ca998 | perfect | ald143 |
| ald144 | 6-14-07 | 9145-I716062 | #3 | ca998 | wrong | ald144 |
| ald145 | 6-14-07 | 9145-I716063 | #1 | ca998 | huge deletion | ald145 |
| ald146 | 6-14-07 | 9145-I716063 | #2 | ca998 | huge deletion | ald146 |
| ald147 | 6-14-07 | 9145-I716063 | #3 | ca998 | silent point mutation, T->A, Val 18.33% -> Val 10.88% | ald147 |
| ald148 | 6-18-07 | 9145-I716061 | #1 | D57 | Perfect (shows beta HbA) | ald148 |
| ald149 | 6-18-07 | 9145-I716061 | #2 | D57 | Perfect (shows alpha HbA) | ald149 |
| ald150 | 6-18-07 | 9145-I716061 | #1 | D58 | Perfect | ald150 |
| ald151 | 6-18-07 | 9145-I716061 | #2 | D58 | Perfect | ald151 |
| ald152 | 6-19-07 | 9145-1154 | #3 | ca998 | Wrong | ald152 |
| ald153 | 6-19-07 | 9145-1154 | #4 | ca998 | Failed | ald153 |
| ald154 | 6-22-07 | 9145-1154 | #1a | ca998 | point mutation K96R | ald154 |
| ald155 | 6-22-07 | 9145-1154 | #1b | ca998 | point mutation K96R | ald155 |
| ald156 | 6-22-07 | 9145-1154 | #1c | ca998 | point mutation K96R | ald156 |
| ald157 | 6-26-07 | 9145-I716070 | #1 | ca998 | Perfect! | ald157 |
| ald158 | 6-26-07 | 9145-I716070 | #2 | ca998 | Perfect! | ald158 |
| ald159 | 6-26-07 | 9145-I716070 | #3 | ca998 | Perfect! | ald159 |
| ald160 | 6-26-07 | pTrc-I716069 | #1 | D57 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald160 |
| ald161 | 6-26-07 | pTrc-I716069 | #1 | D58 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald161 |
| ald162 | 6-26-07 | pTrc-I716069 | #2 | D57 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald162 |
| ald163 | 6-26-07 | pTrc-I716069 | #2 | D58 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald163 |
| ald164 | 6-27-07 | 9145-I716073 | #1 | ca998 | Wrong | ald164 |
| ald165 | 6-27-07 | 9145-I716073 | #2 | ca998 | Wrong | ald165 |
| ald166 | 6-27-07 | 9145-I716073 | #3 | ca998 | Silent point mutation! | ald166 |
| ald167 | 6-27-07 | 9145-I716072 | #1 | ca998 | Wrong | ald167 |
| ald168 | 6-27-07 | 9145-I716072 | #2 | ca998 | Wrong | ald168 |
| ald169 | 6-27-07 | 9145-I716072 | #3 | ca998 | wrong | ald169 |
| ald170 | 6-27-07 | 9145-I716068 | #1 | ca998 | Point deletion | ald170 |
| ald171 | 6-27-07 | 9145-I716068 | #2 | ca998 | Wrong | ald171 |
| ald172 | 6-27-07 | 9145-I716068 | #3 | ca998 | Wrong | ald172 |
| ald173 | 7-01-07 | 9145-I716068 | 1a | ca998 | Perfect | ald173 |
| ald174 | 7-01-07 | 9145-I716068 | 2a | ca998 | Point deletion, but probably not, near the end of read and it's kind of fuzzy | ald174 |
| ald175 | 7-01-07 | 9145-I716068 | 5a | ca998 | Point mutation and is parent vector from the previous step. (No mutation) | ald175 |
| ald176 | 7-01-07 | 9145-I716068 | 6a | ca998 | Perfect | ald176 |
| ald177 | 7-01-07 | 9145-I716068 | 8a | ca998 | Perfect | ald177 |
| ald178 | 7-04-07 | 9145-I716072 | 1 | ca998 | Perfect | ald178 |
| ald179 | 7-04-07 | 9145-I716072 | 2 | ca998 | Perfect | ald179 |
| ald180 | 7-04-07 | 9145-I716072 | 3 | ca998 | Perfect | ald180 |
| ald181 | 7-04-07 | 9145-I716072 | 4 | ca998 | Perfect | ald181 |
| ald182 | 7-04-07 | 9145-I716072 | 5 | ca998 | Wrong | ald182 |
| ald183 | 7-04-07 | 9145-I716072 | 6 | ca998 | Perfect | ald183 |
| ald184 | 7-04-07 | 9145-I716077 | 1 | ca998 | The point mutation changed back, still silent (Didn't "really" change back) otherwise perfect | ald184 |
| ald185 | 7-04-07 | 9145-I716077 | 2 | ca998 | Perfect until the read goes bad (probably should start sequencing backwards too) | ald185 |
| ald186 | 7-04-07 | 9145-I716078 | 1 | ca998 | Perfect | ald186 |
| ald187 | 7-04-07 | 9145-I716078 | 2 | ca998 | Perfect | ald187 |
| ald188 | 7-04-07 | 9145-I716079 | 1 | ca998 | Perfect | ald188 |
| ald189 | 7-04-07 | 9145-I716079 | 2 | ca998 | Perfect until read goes bad. | ald189 |
| ald190 | 07-09-07 | 9145-I716011 | ca998 | Perfect | ald190 | |
| ald191 | 07-09-07 | 9145-I716011 | g00101 | Perfect | ald191 | |
| ald192 | 07-09-07 | 9145-I716071 | ca998 | Wrong, missing alpha subunit | ald192 | |
| ald193 | 07-09-07 | 9145-I716071 | g00101 | Wrong, missing alpha subunit | ald193 | |
| ald194 | 07-09-07 | 9145-I716074 | ca998 | Wrong, missing alpha subunit | ald194 | |
| ald195 | 07-09-07 | 9145-I716074 | g00101 | Wrong, missing alpha subunit | ald195 | |
| ald196 | 07-09-07 | 9145-I716075 | ca998 | Wrong, missing alpha subunit | ald196 | |
| ald197 | 07-09-07 | 9145-I716075 | g00101 | Wrong, missing alpha subunit | ald197 | |
| ald198 | 07-09-07 | 9145-I716076 | ca998 | Wrong, missing alpha subunit | ald198 | |
| ald199 | 07-09-07 | 9145-I716076 | g00101 | Wrong, missing alpha subunit | ald199 | |
| ald200 | 07-09-07 | 9145-I716083 | #2 | D57 | Wrong | ald200 |
| ald201 | 07-09-07 | 9145-I716084 | #2 | D57 | Wrong construct, has providence and the 2X alpha subunit | ald201 |
| ald202 | 07-09-07 | 9145-I716085 | #2 | D57 | Failed read: Multiple Plasmids | ald202 |
| ald203 | 07-09-07 | 9145-I716086 | #2 | D57 | Missing Alpha Subunit | ald203 |
| ald204 | 07-13-07 | 9145-I716080 | ca998 | Perfect | ald204 | |
| ald205 | 07-13-07 | 9145-I716081 | ca998 | Perfect | ald205 | |
| ald206 | 07-13-07 | 9145-I716082 | ca998 | Silent point mutation, so ~Perfect! (Phew!) | ald206 | |
| ald207 | 7-16-07 | 9145-I716074 | ca998 | Beta part is good, but lots of background | ald207 | |
| ald208 | 7-16-07 | 9145-I716074 | g00101 | can see the end of an alpha, but can't tell if its a di-alpha. Terminator perfect | ald208 | |
| ald209 | 7-16-07 | 9145-I716075 | ca998 | Can see perfect beta | ald209 | |
| ald210 | 7-16-07 | 9145-I716075 | g00101 | Can see perfect terminator and the link between the alpha subunits, lots of "point mutations" due to a mixed template, will proceed with this | ald210 | |
| ald211 | 7-16-07 | 9145-I716076 | ca998 | Can see beta perfectly | ald211 | |
| ald212 | 7-16-07 | 9145-I716076 | g00101 | Term and most of alpha present. "point mutations" due to mixed read, but seems good enough to proceed | ald212 | |
| ald213 | 7-17-07 | 9145-I716084 | ca998 | Shows promoter | ald213 | |
| ald214 | 7-17-07 | 9145-I716085 | ca998 | Shows promoter | ald214 | |
| ald215 | 7-17-07 | 9145-I716086 | ca998 | Shows promoter | ald215 | |
| ald216 | 7-17-07 | 9145-I716071 | ca998 | Mixed template, but almost perfect | ald216 | |
| ald217 | 7-17-07 | 9145-I716071 | g00101 | Mixed template, but almost perfect | ald217 | |
| ald218 | 7-18-07 | 9145-I716083 | ca998 | Wrong, sucka. (I think I overloadded the lane, will do again) | ald218 | |
| ald219 | 7-19-07 | 9145-I716084 | 1c | ca998 | Gene is gone?!! | ald219 |
| ald220 | 7-19-07 | 9145-I716084 | 1c | g00101 | Gene is gone?!! | ald220 |
| ald221 | 7-19-07 | 9145-I716085 | 1c | ca998 | Very badly mixed read | ald221 |
| ald222 | 7-19-07 | 9145-I716085 | 1c | g00101 | Very badly mixed read | ald222 |
| ald223 | 7-19-07 | 9145-I716086 | 1c | ca998 | Very badly mixed read | ald223 |
| ald224 | 7-19-07 | 9145-I716086 | 1c | g00101 | Very badly mixed read | ald224 |
| ald225 | 7-23-07 | 9145-I716083 | 3 | ca998 | Perfect | ald225 |
| ald226 | 7-23-07 | 9145-I716083 | 4 | ca998 | Perfect | ald226 |
| ald227 | 7-23-07 | 9145-I716084 | 1 | ca998 | Silent Point Mutation | ald227 |
| ald228 | 7-23-07 | 9145-I716084 | 3 | ca998 | Damn wrong | ald228 |
| ald229 | 7-23-07 | 9145-I716085 | 1 | ca998 | Point mutation! Damn it!! V->A | ald229 |
| ald230 | 7-23-07 | 9145-I716086 | 1 | ca998 | Perfecto! | ald230 |
| ald231 | 7-23-07 | 9145-I716086 | 3 | ca998 | Damn wrong | ald231 |
| ald232 | 7-23-07 | 9145-I716086 | 2z | ca998 | Perfecto! | ald232 |
| ald233 | 7-23-07 | 9145-I716083 | 3 | g00101 | Perfect | ald233 |
| ald234 | 7-23-07 | 9145-I716083 | 4 | g00101 | Perfect | ald234 |
| ald235 | 7-23-07 | 9145-I716084 | 1 | g00101 | Silent point mutation | ald235 |
| ald236 | 7-23-07 | 9145-I716084 | 3 | g00101 | Damn wrong | ald236 |
| ald237 | 7-23-07 | 9145-I716085 | 1 | g00101 | Point mutation! Damn it!! V->A | ald237 |
| ald238 | 7-23-07 | 9145-I716086 | 1 | g00101 | Perfecto! | ald238 |
| ald239 | 7-23-07 | 9145-I716086 | 3 | g00101 | Damn wrong | ald239 |
| ald240 | 7-23-07 | 9145-I716086 | 2z | g00101 | Perfecto! | ald240 |
| ald241 | 7-26-07 | 9145-I716085 #1 cpcr | ca998 | Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together | ald241 | |
| ald242 | 7-26-07 | 9145-I716085 #2 cpcr | ca998 | Can see link between dialpha and whole beta, looks perfect | ald242 | |
| ald243 | 7-26-07 | 9145-I716085 #1 cpcr | g00101 | Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together | ald243 | |
| ald244 | 7-26-07 | 9145-I716085 #2 cpcr | g00101 | Can see link between dialpha and whole beta, looks perfect | ald244 | |
| ald245 | 7-30-07 | petDUET-I716091 | 1 | AD0010F | ald245 | |
| ald246 | 7-30-07 | petDUET-I716091 | 1 | AD0011F | ald246 | |
| ald247 | 7-30-07 | petDUET-I716093 | 2-1 | AD0010F | ald247 | |
| ald248 | 7-30-07 | petDUET-I716094 | 1-3 | AD0010F | ald248 | |
| ald249 | 7-30-07 | petDUET-I716094 | 1-3 | AD0011F | ald249 | |
| ald250 | ald250 | |||||
| ald251 | ald251 | |||||
| ald252 | ald252 | |||||
| ald253 | ald253 | |||||
| ald254 | ald254 | |||||
| ald255 | ald255 | |||||
| ald256 | ald256 | |||||
| ald257 | ald257 | |||||
| ald258 | ald258 | |||||
| ald259 | ald259 | |||||
| ald260 | ald260 | |||||
| ald261 | ald261 | |||||
| ald262 | ald262 | |||||
| ald263 | ald263 | |||||
| ald264 | ald264 | |||||
| ald265 | ald265 | |||||
| ald266 | ald266 | |||||
| ald267 | ald267 | |||||
| ald268 | ald268 | |||||
| ald269 | ald269 | |||||
| ald270 | ald270 | |||||
| ald271 | ald271 | |||||
| ald272 | ald272 | |||||
| ald273 | ald273 | |||||
| ald274 | ald274 | |||||
| ald275 | ald275 | |||||
| ald276 | ald276 | |||||
| ald277 | ald277 | |||||
| ald278 | ald278 | |||||
| ald279 | ald279 | |||||
| ald280 | ald280 | |||||
| ald281 | ald281 | |||||
| ald282 | ald282 | |||||
| ald283 | ald283 | |||||
| ald284 | ald284 | |||||
| ald285 | ald285 | |||||
| ald286 | ald286 | |||||
| ald287 | ald287 | |||||
| ald288 | ald288 | |||||
| ald289 | ald289 | |||||
| ald290 | ald290 | |||||
| ald291 | ald291 | |||||
| ald292 | ald292 | |||||
| ald293 | ald293 | |||||
| ald294 | ald294 | |||||
| ald295 | ald295 | |||||
| ald296 | ald296 | |||||
| ald297 | ald297 | |||||
| ald298 | ald298 | |||||
| ald299 | ald299 | |||||
| ald300 | ald300 | |||||
| ald301 | ald301 | |||||
| ald302 | ald302 | |||||
| ald303 | ald303 | |||||
| ald304 | ald304 | |||||
| ald305 | ald305 | |||||
| ald306 | ald306 | |||||
| ald307 | ald307 | |||||
| ald308 | ald308 | |||||
| ald309 | ald309 | |||||
| ald310 | ald310 | |||||
| ald311 | ald311 | |||||
| ald312 | ald312 | |||||
| ald313 | ald313 | |||||
| ald314 | ald314 | |||||
| ald315 | ald315 | |||||
| ald316 | ald316 | |||||
| ald317 | ald317 | |||||
| ald318 | ald318 | |||||
| ald319 | ald319 |
