Mechanism I
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We propose to link the riboflavin operon of Bacillus subtilis to plasmid puc18-ADH1 p+t(figure1). ADH1p(560bp) is a strong promoter of Yeast and ADH1t is the terminater corresponding to this promoter. The initial carrier of puc18-ADH1 p+t is PUC18. The construction of this plamid is: | We propose to link the riboflavin operon of Bacillus subtilis to plasmid puc18-ADH1 p+t(figure1). ADH1p(560bp) is a strong promoter of Yeast and ADH1t is the terminater corresponding to this promoter. The initial carrier of puc18-ADH1 p+t is PUC18. The construction of this plamid is: | ||
#Digest ADH1p with XbaI and BAMHI and link it to the relevant site of PUB18; | #Digest ADH1p with XbaI and BAMHI and link it to the relevant site of PUB18; | ||
Revision as of 09:31, 5 August 2007
We propose to link the riboflavin operon of Bacillus subtilis to plasmid puc18-ADH1 p+t(figure1). ADH1p(560bp) is a strong promoter of Yeast and ADH1t is the terminater corresponding to this promoter. The initial carrier of puc18-ADH1 p+t is PUC18. The construction of this plamid is:
- Digest ADH1p with XbaI and BAMHI and link it to the relevant site of PUB18;
- Digest ADH1t with KpnI and SacI and link it to the relevant site of PUC18.
Our main work is :
