Melbourne/Blue Photosensor Background
From 2007.igem.org
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[[Melbourne/Blue Photosensor Background/ComP/AA|AA]], | [[Melbourne/Blue Photosensor Background/ComP/AA|AA]], | ||
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | |||
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@927) deleted | [[Melbourne/primary_Restriction_enzymes|SpeI]] (@927) deleted | ||
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@1902) inserted | [[Melbourne/primary_Restriction_enzymes|HaeII]] (@1902) inserted | ||
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[[Melbourne/Blue Photosensor Background/ComA/AA|AA]], | [[Melbourne/Blue Photosensor Background/ComA/AA|AA]], | ||
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | |||
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted | [[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted | ||
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted | [[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted | ||
Revision as of 11:50, 6 August 2007
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Contents |
Preliminaries
SopII
sensory rhodopsin II
DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- HaeII (@168) present
HtrII
sensory rhodopsin II transducer
DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3702851&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum genbank]
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion
DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA,
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP
two-component sensor histidine kinase
DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
SpeI (@927) deleted HaeII (@1902) inserted
ComA
sensory rhodopsin II transducer
DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
SpeI (@12) deleted HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
