NYMU Taipei/Lab Notes/2007 8 6&7
From 2007.igem.org
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<h3>Lab schedule</h3> | <h3>Lab schedule</h3> | ||
* mouse insulin gene cloning (delay to 8/7) | * mouse insulin gene cloning (delay to 8/7) | ||
| - | ** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus] | + | ** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus], [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=142350788&dopt=GenBank&WebEnv=04iJq7mV5tF1N_LSoJWAy8uc-0i_vXvfkGc3KMxcxnrIPG9K-LDVOuYkZWjzh6BpujGcDGNGQk0k9I%40D4770B146B72B990_0041SID&WebEnvRq=1 MN_008387.3] |
| - | *** primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN) | + | *** primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN; 341-403: GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC) |
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | **** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | ||
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | **** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | ||
Revision as of 14:18, 6 August 2007
Lab schedule
- mouse insulin gene cloning (delay to 8/7)
- primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus], [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=142350788&dopt=GenBank&WebEnv=04iJq7mV5tF1N_LSoJWAy8uc-0i_vXvfkGc3KMxcxnrIPG9K-LDVOuYkZWjzh6BpujGcDGNGQk0k9I%40D4770B146B72B990_0041SID&WebEnvRq=1 MN_008387.3]
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN; 341-403: GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC)
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
- primer for B chain
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN; 341-403: GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC)
- primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus], [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=142350788&dopt=GenBank&WebEnv=04iJq7mV5tF1N_LSoJWAy8uc-0i_vXvfkGc3KMxcxnrIPG9K-LDVOuYkZWjzh6BpujGcDGNGQk0k9I%40D4770B146B72B990_0041SID&WebEnvRq=1 MN_008387.3]
- purchase of BCRC human insulin clone (ordered not paid)
- antibiotics LB plate preparation
- ampicillin
- kanamycin
