From 2007.igem.org

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		+	==July 07 2007==
		+	Attempted Transformation of 4 Biobricks
		+	1. BBa_J5526 - Plate 3 well 6F - RFP
		+	2. BBa_I13522 - Plate 2 well 15H - GFP
		+	3. BBa_R0011 - Plate 1 well 7M - LacI inhibited promoter
		+	4. BBa_PO440 - Plate 1 well 21K - GFP repressor
		+
		+	Protocol
		+	1. Add 15uL deionized H2O to well
		+	2. Add 1 uL plasmid to 25uL DH5alpha
		+	3. Incubate on ice for 30min
		+	4. Heat shock without shaking for 20sec
		+	5. Place on ice for 2min
		+	6. Add 0.5mL LB, incubate for 1hr
		+	7. Plate 100uL on amp+ LB plates, incubate overnight at 37 C
		+
		+
		+
	===July 24===		===July 24===
	Note book created		Note book created

---

Revision as of 17:14, 25 August 2007

Contents 1 July 07 2007 1.1 July 24 1.2 July 25 1.3 July 27

July 07 2007

Attempted Transformation of 4 Biobricks

 1. BBa_J5526 - Plate 3 well 6F - RFP
 2. BBa_I13522 - Plate 2 well 15H - GFP
 3. BBa_R0011 - Plate 1 well 7M - LacI inhibited promoter
 4. BBa_PO440 - Plate 1 well 21K - GFP repressor

Protocol

 1. Add 15uL deionized H2O to well
 2. Add 1 uL plasmid to 25uL DH5alpha
 3. Incubate on ice for 30min
 4. Heat shock without shaking for 20sec
 5. Place on ice for 2min
 6. Add 0.5mL LB, incubate for 1hr
 7. Plate 100uL on amp+ LB plates, incubate overnight at 37 C

July 24

Note book created

July 25

  * GFP repressor (TetR) and LacI inhibited promoter transformed successfully
  * RFP with promoter, RBS, and terminators transformed but does not emit RF 
    under UV light and plasmid too small, will have to build from five subparts.
  * GFP, LacI, and four subparts of RFP left to go

July 27