McGill
From 2007.igem.org
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'''Team 2 - Fluorescence Complimentation''' | '''Team 2 - Fluorescence Complimentation''' | ||
| - | This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein | + | This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate. |
Revision as of 20:45, 15 May 2007
Contents |
Message Board (temporary)
I'll chop up Adam's PDF on my break today to put it into the Protocols section. Tim
Hello Tim,
we need a lab notebook page, protocols page and team members page, etc.
PS: Everyone, get a lab notebook! We're making our cells chemically competent tomorrow so if you want to see/help out then show up around 10:30. Horiavulpe 16:41, 14 May 2007 (EDT)
Note from iGEM: Please use the format "McGill/Page Name" for all the pages your team uses. I fixed
teh Lab Protocols, the Lab Notebook, and the Team Roster links. Thanks, - Randy
In the Lab
The Team
Project Overview
Team 1 - Repressilator Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations. We will also test the effect of extracellularly adding autoinducer on synchronization.
Team 2 - Fluorescence Complimentation This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate.
