Week 3
From 2007.igem.org
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*We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed: | *We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed: | ||
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up | -BBa_ J04430 to test the GFP fluorescence detection with our experimental set up | ||
| + | |||
-BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project. | -BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project. | ||
| - | + | ||
*BBa_J04500, the PLac promoter inducible by IPTG; | *BBa_J04500, the PLac promoter inducible by IPTG; | ||
*BBa_J04631, the GFP (+LVA) protein. | *BBa_J04631, the GFP (+LVA) protein. | ||
Revision as of 13:34, 9 August 2007
- 07/17/07
- We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up
-BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
- BBa_J04500, the PLac promoter inducible by IPTG;
- BBa_J04631, the GFP (+LVA) protein.
- We straked on plates with the right antibiotic.
- 7/18/07
- We performed a test for GFP induction with IPTG. We picked a colony from the J04430 plate and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.
- We picked a colony from 07/17 plates and inoculated in 5 ml of LB medium from each plate and incubated overnight.
