From 2007.igem.org

Revision as of 11:26, 13 August 2007 (view source) Nicolas C. (Talk | contribs) ← Older edit		Revision as of 11:29, 13 August 2007 (view source) Nicolas C. (Talk | contribs) (→Digestions) Newer edit →
Line 6:		Line 6:
	* L10 (lox71 BV) Spe Pst		* L10 (lox71 BV) Spe Pst
	* MP8.1 (gfp tri-part FI) Eco Spe		* MP8.1 (gfp tri-part FI) Eco Spe
		+
		+	digestion mix:
		+	* 25µl plasmid
		+	* 1µl BSA
		+	* 10 µl NEB2
		+	* 60µl H2O
		+	* 2µl Enzyme1
		+	* 2µl Enzyme2

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Revision as of 11:29, 13 August 2007

Digestions

• L1.2 (AraC-pBAD BV) Spe Pst
• L6.1 (rbs-CRE) Eco Pst
• L6.2 (rbs-CRE) Xba Pst
• L9 (lox66 FV) Eco Xba
• L10 (lox71 BV) Spe Pst
• MP8.1 (gfp tri-part FI) Eco Spe

digestion mix:

• 25µl plasmid
• 1µl BSA
• 10 µl NEB2
• 60µl H2O
• 2µl Enzyme1
• 2µl Enzyme2