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	* Gel Electrophoresis of R0040, R0051, Q04121, C0053		* Gel Electrophoresis of R0040, R0051, Q04121, C0053
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-	| * Tim (from 17:30 until it's done)<br> Martin<br> Christos (11:00-13:00, 18:00-00:00)	+	|Morning Shift (start 9am): <br> Markus, Tim <br> <br> Evening Shift (start 5pm):<br> Martin, Christos
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	| Wed, 15. Aug. 2007		| Wed, 15. Aug. 2007

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Revision as of 19:21, 13 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

1. [http://partsregistry.org/Part:BBa_B0034 B0034]
2. [http://partsregistry.org/Part:BBa_R0062 R0062]
3. [http://partsregistry.org/Part:BBa_R0053 R0053]
4. [http://partsregistry.org/Part:BBa_J23100 J23100]
5. [http://partsregistry.org/Part:BBa_J37033 J37033]
6. [http://partsregistry.org/Part:BBa_E0434 E0434]
7. [http://partsregistry.org/Part:BBa_B0015 B0015]
8. [http://partsregistry.org/Part:BBa_Q04400 Q04400]
9. [http://partsregistry.org/Part:BBa_R0010 R0010]
10. [http://partsregistry.org/Part:BBa_E0422 E0422]
11. [http://partsregistry.org/Part:BBa_R0040 R0040]
12. [http://partsregistry.org/Part:BBa_R0051 R0051]
13. [http://partsregistry.org/Part:BBa_Q04121 Q04121]
14. [http://partsregistry.org/Part:BBa_C0053 C0053]
15. [http://partsregistry.org/Part:BBa_Q04510 Q4510]

Week 1

Date	TODO's	Completed	People
Mon, 06. Aug. 2007
Tue, 07. Aug. 2007
Wed, 08. Aug. 2007
Thu, 09. Aug. 2007
Fri, 10. Aug. 2007		7 working parts/plasmids (step after "DIGESTS"): (B0034, R0062, R0053, E0434, B0015, R0010, E0422) 4 parts/plasmids minipreped: (R0040, R0051, Q04121, C0053)	Christos Markus Stefan
Sat, 11. Aug. 2007
Sun, 12. Aug. 2007 labwork cancelled			Christos Joe Martin

Week 2

Date	TODO's	Completed	People
Mon, 13. Aug. 2007 start at 3 pm	Prepare competent cells Transformations of J23100, J37033, Q04400, Q04510 Control Restrictions (step after "MINIPREP") R0040, R0051, Q04121, C0053	Overnight culture (Top 10 cells) Control Restrictions (didn't work)	Martin Markus Christos Tim
Tue, 14. Aug. 2007	Morning Shift: Start Preparing competent cells for J23100, J37033, Q04400, Q04510 Control Restrictions (step after "MINIPREP") R0040, R0051, Q04121, C0053 Evening Shift: Transformations of J23100, J37033, Q04400, Q04510 Gel Electrophoresis of R0040, R0051, Q04121, C0053		Morning Shift (start 9am): Markus, Tim Evening Shift (start 5pm): Martin, Christos
Wed, 15. Aug. 2007	Miniprep of competent cells (J23100, J37033, Q04400, Q04510) Ligation (step: "LINK ASSEMBLY"): R0053 + E0422 R0010 + E0422 R0010 + E0434 S/P: R0053, R0010 X/P: E0422, E0434		* Tim (from 17:30 until it's done) Martin Christos (11:00-13:00, 18:00-00:00)
Thu, 16. Aug. 2007			Christos (11:00-13:00, 18:00-00:00) Martin
Fri, 17. Aug. 2007			Martin Nan (from 18:00 till 00:00)
Sat, 18. Aug. 2007			Martin Nan
Sun, 19. Aug. 2007			Martin Nan