Paris/August 14
From 2007.igem.org
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| + | == Transformation of a lox-KmR-lox strain with a pBAD-CRE plasmid == | ||
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| + | Francois Xavier Barre gave us the strain FX85 which as a lox-Km-lox in its chromosome. We will prepare CaCl2 competent cells of FX85 and transform it with a plasmid bearing pBAD-CRE. This will allow us measuring the excision frequency of the CRE/LOX system with different levels of induction | ||
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==MiniPrep and glycerol stock == | ==MiniPrep and glycerol stock == | ||
* <bbpart>J61002</bbpart> | * <bbpart>J61002</bbpart> | ||
* <bbpart>J61025</bbpart> | * <bbpart>J61025</bbpart> | ||
* <bbpart>B0015</bbpart> | * <bbpart>B0015</bbpart> | ||
Revision as of 10:53, 14 August 2007
Transformation of a lox-KmR-lox strain with a pBAD-CRE plasmid
Francois Xavier Barre gave us the strain FX85 which as a lox-Km-lox in its chromosome. We will prepare CaCl2 competent cells of FX85 and transform it with a plasmid bearing pBAD-CRE. This will allow us measuring the excision frequency of the CRE/LOX system with different levels of induction
