NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 8: Line 8:
!Description
!Description
|-
|-
-
|INS_A 5'
+
|M_INS_A 5'
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">ATTGTAGATCAGTGCTGCAC</font>
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">ATTGTAGATCAGTGCTGCAC</font>
-
|51
+
|51 bp
|
|
|EcoRI, XbaI
|EcoRI, XbaI
Line 17: Line 17:
|-
|-
|-
|-
-
|INS_A 3'
+
|M_INS_A 3'
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GAAATACCTTGCTTCTTTTCG</font>
-
|55
+
|55 bp
|
|
|SpeI, PstI
|SpeI, PstI
|63 bp
|63 bp
|
|
 +
|-
 +
|-
 +
|RcsC 5'
 +
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGAT</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
 +
|51 bp
 +
|55.92
 +
|EcoRI, XbaI
 +
|1396 bp
 +
|
 +
|-
 +
|-
 +
|RcsC 3'-2
 +
|<font color="green">AAGTATAC</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
 +
|29 bp
 +
|59.63
 +
|NdeI
 +
|1396 bp
 +
|Two extra Adenosine was added to the 5' side of the NdeI cutting site.
|-
|-

Revision as of 08:41, 17 August 2007

Name Sequence Length Tm Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC 51 bp EcoRI, XbaI 63 bp
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAAATACCTTGCTTCTTTTCG 55 bp SpeI, PstI 63 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTGCAGTAGTTCTCCAGCT 51 bp 55.92 EcoRI, XbaI 1396 bp
RcsC 3'-2 AAGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1396 bp Two extra Adenosine was added to the 5' side of the NdeI cutting site.
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