NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
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|M_INS_A 3' | |M_INS_A 3' | ||
| - | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red"> | + | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font> |
|56 bp | |56 bp | ||
| - | | | + | | |
|SpeI, PstI | |SpeI, PstI | ||
|63 bp | |63 bp | ||
| Line 27: | Line 27: | ||
|- | |- | ||
|RcsC 5' | |RcsC 5' | ||
| - | |<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGAT</font><font color="red"> | + | |<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGAT</font><font color="red">GAAATACCTTGCTTCTTTTCG</font> |
|51 bp | |51 bp | ||
|55.92 | |55.92 | ||
Revision as of 08:49, 17 August 2007
| Name | Sequence | Length | Tm | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC | 51 bp | EcoRI, XbaI | 63 bp | ||
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 56 bp | SpeI, PstI | 63 bp | ||
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1396 bp | |
| RcsC 3'-2 | AAGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1396 bp | Two extra Adenosine was added to the 5' side of the NdeI cutting site. |
