NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 9: Line 9:
|-
|-
|M_INS_A 5'
|M_INS_A 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">ATTGTAGATCAGTGCTGCAC</font>
+
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
|51 bp
|51 bp
|
|
|EcoRI, XbaI
|EcoRI, XbaI
|63 bp
|63 bp
-
|
+
|Potential dimer formation! Need to design a new one.
|-
|-
|-
|-
Line 23: Line 23:
|SpeI, PstI
|SpeI, PstI
|63 bp
|63 bp
 +
|Potential dimer formation! Need to design a new one.
 +
|-
 +
|M_INS_B 5'
 +
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTG</font>
 +
|51 bp
 +
|60.03
 +
|EcoRI, XbaI
 +
|63 bp
 +
|Potential dimer formation! Need to design a new one.
 +
|-
 +
|-
 +
|M_INS_B 3'
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
 +
|55 bp
 +
|55.86
 +
|SpeI, PstI
 +
|90 bp
|
|
|-
|-

Revision as of 09:07, 17 August 2007

Name Sequence Length Tm Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTTGCAGTAGTTCTCCAGCT 51 bp EcoRI, XbaI 63 bp Potential dimer formation! Need to design a new one.
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp SpeI, PstI 63 bp Potential dimer formation! Need to design a new one.
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG 51 bp 60.03 EcoRI, XbaI 63 bp Potential dimer formation! Need to design a new one.
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 90 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1396 bp
RcsC 3'-2 AAGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1396 bp Two extra Adenosine was added to the 5' side of the NdeI cutting site.
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