NYMU Taipei/Primer List
From 2007.igem.org
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|M_INS_B 5' | |M_INS_B 5' | ||
| - | |<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red"> | + | |<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font> |
|51 bp | |51 bp | ||
|60.03 | |60.03 | ||
Revision as of 09:11, 17 August 2007
| Name | Sequence | Length | Tm | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTTGCAGTAGTTCTCCAGCT | 51 bp | EcoRI, XbaI | 63 bp | Potential dimer formation! Need to design a new one. | |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 55 bp | SpeI, PstI | 63 bp | Potential dimer formation! Need to design a new one. | |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 51 bp | 60.03 | EcoRI, XbaI | 63 bp | Potential dimer formation! Need to design a new one. |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA | 55 bp | 55.86 | SpeI, PstI | 90 bp | |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1396 bp | |
| RcsC 3'-2 | AAGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1396 bp | Two extra adenosine was added to the 5' side of the NdeI cutting site. |
